| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOXB13 |
| Uniprot No | Q92826 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-284aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEPGNYA TLDGAKDIEG LLGAGGGRNL VAHSPLTSHP AAPTLMPAVN YAPLDLPGSA EPPKQCHPCP GVPQGTSPAP VPYGYFGGGY YSCRVSRSSL KPCAQAATLA AYPAETPTAG EEYPSRPTEF AFYPGYPGTY QPMASYLDVS VVQTLGAPGE PRHDSLLPVD SYQSWALAGG WNSQMCCQGE QNPPGPFWKA AFADSSGQHP PDACAFRRGR KKRIPYSKGQ LRELEREYAA NKFITKDKRR KISAATSLSE RQITIWFQNR RVKEKKVLAK VKNSATP |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HOXB13重组蛋白的3篇参考文献及其摘要概括：
1. **"HOXB13 suppresses prostate cancer metastasis through regulation of androgen receptor-dependent transcriptional networks"**
*作者：Zhang, X., et al.*
摘要：该研究通过重组HOXB13蛋白的体外实验，揭示了其通过调控雄激素受体(AR)的靶基因转录，抑制前列腺癌细胞侵袭和转移的分子机制。
2. **"Functional characterization of recombinant HOXB13 in colorectal cancer progression"**
*作者：Lee, S., & Kim, J.H.*
摘要：研究者利用大肠癌细胞模型，证明重组HOXB13蛋白通过抑制Wnt/β-catenin信号通路，降低肿瘤细胞增殖和迁移能力，提示其潜在抑癌作用。
3. **"Production and purification of recombinant HOXB13 for structural analysis"**
*作者：Gupta, R., et al.*
摘要：本文详细描述了在大肠杆菌系统中高效表达和纯化重组HOXB13蛋白的方法，并通过X射线晶体学解析了其DNA结合结构域的三维结构，为靶向药物设计提供依据。
(注：以上文献信息为模拟示例，实际引用需以真实文献为准。)
**Background of HOXB13 Recombinant Protein**
HOXB13. a member of the homeobox (HOX) gene family, encodes a transcription factor critical in regulating developmental patterning, cell differentiation, and tissue homeostasis. HOX proteins, characterized by a conserved DNA-binding homeodomain, play pivotal roles in embryonic development, particularly in establishing anterior-posterior body axis identity. HOXB13 is specifically implicated in the development and function of the prostate, gastrointestinal tract, and urogenital system. Its expression persists in certain adult tissues, where it modulates cellular proliferation, apoptosis, and lineage-specific gene programs.
Dysregulation of HOXB13 is linked to multiple cancers, notably prostate cancer, where it acts as both a tumor suppressor and oncogene depending on context. For instance, germline mutations (e.g., rs138213197) in HOXB13 are associated with hereditary prostate cancer risk, while overexpression correlates with advanced tumor stages. HOXB13 interacts with androgen receptor signaling, influencing hormone-responsive gene networks, and may promote metastasis by regulating extracellular matrix remodeling.
Recombinant HOXB13 protein, produced via expression systems (e.g., *E. coli* or mammalian cells), retains the functional homeodomain for DNA binding and protein interactions. Purification tags (e.g., His-tag) facilitate isolation for experimental use. This engineered protein enables *in vitro* studies, such as elucidating DNA-binding motifs, mapping interaction partners (e.g., MEIS co-factors), and screening small-molecule inhibitors targeting HOXB13-driven pathways.
Beyond basic research, HOXB13 recombinant protein holds translational potential. It aids in developing diagnostic assays for HOXB13-associated cancers and serves as a tool to explore therapeutic strategies, including disrupting oncogenic HOXB13 activity. Ongoing studies also investigate its role in tissue regeneration and stem cell regulation, underscoring its multifaceted biological relevance.
In summary, HOXB13 recombinant protein is a vital resource for dissecting developmental and disease mechanisms, bridging molecular insights to clinical applications.
×
