| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOMER3 |
| Uniprot No | Q9NSC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-361aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMMSTAR EQPIFSTRAH VFQIDPATKR NWIPAGKHAL TVSYFYDATR NVYRIISIGG AKAIINSTVT PNMTFTKTSQ KFGQWADSRA NTVYGLGFAS EQHLTQFAEK FQEVKEAARL AREKSQDGGE LTSPALGLAS HQVPPSPLVS ANGPGEEKLF RSQSADAPGP TERERLKKML SEGSVGEVQW EAEFFALQDS NNKLAGALRE ANAAAAQWRQ QLEAQRAEAE RLRQRVAELE AQAASEVTPT GEKEGLGQGQ SLEQLEALVQ TKDQEIQTLK SQTGGPREAL EAAEREETQQ KVQDLETRNA ELEHQLRAME RSLEEARAER ERARAEVGRA AQLLDVSLFE LSELREGLAR LAEAAP |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HOMER3重组蛋白的3篇参考文献示例(文献为假设性描述，供参考)：
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1. **文献名称**: *"Recombinant expression and functional characterization of HOMER3 in neuronal signaling complexes"*
**作者**: Smith J, Brown A, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化重组HOMER3蛋白，验证其与代谢型谷氨酸受体mGluR5的相互作用，证明HOMER3作为支架蛋白在神经元突触后信号复合体组装中的关键作用。
2. **文献名称**: *"Structural insights into the scaffolding protein HOMER3 revealed by X-ray crystallography"*
**作者**: Johnson R, Lee C.
**摘要**: 利用重组HOMER3蛋白的EVH1结构域进行结晶和X射线衍射分析，解析其三维结构，揭示了HOMER3通过特异性结合富含脯氨酸的肽段调控下游信号通路的分子机制。
3. **文献名称**: *"HOMER3 regulates TRPC channel activity through recombinant protein interaction studies"*
**作者**: Chen L, Wang H, et al.
**摘要**: 通过体外重组HOMER3与TRPC离子通道的共纯化实验，结合电生理记录，证实HOMER3通过直接结合调控TRPC通道的门控特性，影响细胞内钙信号传递。
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注：以上文献信息为示例，实际研究中请根据具体需求检索PubMed、Web of Science等数据库获取真实文献。
HOMER3 is a member of the HOMER protein family, which comprises scaffold proteins critical for organizing synaptic signaling complexes in the central nervous system. These proteins are evolutionarily conserved and play pivotal roles in modulating synaptic plasticity, receptor trafficking, and intracellular calcium signaling. HOMER3. encoded by the *HOMER3* gene in humans, is primarily expressed in the brain and interacts with metabotropic glutamate receptors (mGluRs), inositol trisphosphate receptors (IP3Rs), and other postsynaptic density proteins. Its structure includes an N-terminal Enabled/VASP homology 1 (EVH1) domain, which binds proline-rich motifs in target proteins, and a C-terminal coiled-coil domain enabling multimerization. This oligomerization allows HOMER3 to form cross-linked networks, facilitating the assembly of macromolecular complexes that regulate neuronal excitability and signaling dynamics.
Recombinant HOMER3 protein is engineered for in vitro studies to dissect its biochemical and functional properties. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant HOMER3 retains binding specificity to its partners, making it invaluable for investigating synaptic protein interactions, calcium homeostasis, and downstream signaling cascades. Researchers use it to explore HOMER3’s role in neurological disorders, such as addiction, epilepsy, and neurodegenerative diseases, where disrupted scaffolding functions may contribute to pathogenesis. Additionally, recombinant HOMER3 aids in drug discovery by serving as a target for compounds aiming to modulate synaptic plasticity or calcium signaling. Its applications extend to structural studies (e.g., crystallography) and functional assays (e.g., pull-down experiments) to map interaction domains or validate genetic mutations. Overall, recombinant HOMER3 is a vital tool for advancing our understanding of synaptic biology and developing therapeutic strategies for brain disorders.
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