| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | esaA |
| Uniprot No | P0C049 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 483-821aa |
| 氨基酸序列 | DPHFNFEGDIKINGKKYDIKDQSVQLDTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVTLNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVINDMESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFAEEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQVDNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKAFMSNPVQKKNLENVLANNGNTDV |
| 预测分子量 | 46.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于esaA重组蛋白的3篇文献示例(注：部分内容基于模拟数据，实际文献请通过学术数据库查询)：
1. **《Structural characterization of the EsaA protein from Staphylococcus aureus》**
*作者：Smith J, et al.*
摘要：本研究通过重组表达纯化了金黄色葡萄球菌EsaA蛋白，利用X射线晶体学解析其三维结构，揭示了其与细菌Type VII分泌系统相互作用的潜在机制，为靶向治疗提供结构基础。
2. **《Functional analysis of EsaA in bacterial pathogenesis》**
*作者：Lee C, et al.*
摘要：通过构建esaA基因缺失突变体及重组蛋白回补实验，证明EsaA在病原菌分泌毒力因子中的关键作用，并发现其重组蛋白可诱导宿主免疫反应，提示其疫苗开发潜力。
3. **《Heterologous expression and purification of recombinant EsaA for antibody production》**
*作者：Wang Y, et al.*
摘要：优化大肠杆菌表达系统制备高纯度重组EsaA蛋白，成功制备多克隆抗体，验证其在临床菌株检测中的应用价值，为快速诊断试剂开发奠定基础。
提示：建议通过PubMed或Google Scholar搜索“esaA recombinant protein”或结合具体物种名称(如“Staphylococcus aureus EsaA”)获取真实文献。
EsaA is a bacterial protein implicated in virulence and secretion processes, particularly within pathogenic species such as *Staphylococcus aureus*. It belongs to the ESAT-6 (Early Secreted Antigenic Target-6) family of proteins, which are small, secreted virulence factors critical for host-pathogen interactions. EsaA is associated with the Type VII secretion system (T7SS), a specialized machinery that exports effector proteins across the bacterial cell envelope. These effectors often play roles in disrupting host immune responses, nutrient acquisition, or interbacterial competition. In *S. aureus*, EsaA is encoded within the *ess* (ESAT-6 secretion system) locus and is essential for the secretion of ESAT-6-like proteins, which contribute to bacterial survival and pathogenesis during infection.
Recombinant EsaA protein is produced through genetic engineering, typically by cloning the *esaA* gene into expression vectors (e.g., *E. coli*), followed by purification using affinity chromatography. Its recombinant form enables detailed structural and functional studies, including elucidating its role in T7SS assembly or substrate recognition. Researchers also explore its potential as a diagnostic antigen or vaccine candidate due to its immunogenic properties. Additionally, studying EsaA interactions with host cells provides insights into bacterial evasion mechanisms, aiding the development of anti-infective therapies. Despite progress, questions remain about its precise molecular mechanisms and regulatory pathways, driving ongoing research to clarify its contribution to bacterial pathogenicity and adaptability.
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