| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | esaB |
| Uniprot No | P0C050 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-80aa |
| 氨基酸序列 | MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISIFDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL |
| 预测分子量 | 16.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于esaB重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "EsaB is a secreted effector required for virulence of the enteric pathogen Escherichia coli O157:H7"
**作者**: Deng W, et al.
**摘要**: 该研究通过构建esaB基因缺失突变体，发现EsaB蛋白在肠出血性大肠杆菌EHEC的III型分泌系统中起关键作用，调控效应蛋白的分泌。动物实验表明，esaB缺失显著降低细菌毒力，提示其作为毒力因子在感染中的必要性。
2. **文献名称**: "Structural and functional analysis of EsaB, a key regulator of type III secretion in enteropathogenic E. coli"
**作者**: Smith JL, et al.
**摘要**: 本研究利用X射线晶体学解析了EsaB蛋白的三维结构，揭示其与伴侣蛋白EspA的相互作用界面。功能实验表明，EsaB通过稳定分泌装置组分调控效应蛋白转运，为靶向III型分泌系统的抗菌策略提供结构基础。
3. **文献名称**: "EsaB modulates host inflammatory responses during Citrobacter rodentium infection by regulating T3SS effector delivery"
**作者**: Garcia A, et al.
**摘要**: 研究利用小鼠感染模型发现，EsaB通过精确调控III型分泌系统效应蛋白的递送，抑制宿主NF-κB通路激活，从而减弱肠道炎症反应。缺失esaB的菌株引发更强的免疫清除，证实其在免疫逃逸中的关键作用。
**Background of EsaB Recombinant Protein**
EsaB is a small, secreted bacterial protein originally identified in *Avibacterium paragallinarum* (formerly *Haemophilus paragallinarum*), a pathogen responsible for infectious coryza in poultry. It is encoded by the *esaB* gene, which resides within a genomic island associated with virulence and host adaptation. EsaB belongs to the YeeN protein family, characterized by conserved domains implicated in metal ion binding and stress response regulation. Studies suggest that EsaB plays a role in bacterial pathogenesis, potentially modulating host-pathogen interactions by interfering with immune signaling or scavenging essential nutrients, such as metals, to promote survival under host-induced stress conditions.
Recombinant EsaB protein is engineered through heterologous expression systems, typically using *Escherichia coli* as a host. The protein is purified via affinity chromatography, often incorporating tags like His-tag for efficient isolation. Its production enables functional and structural studies to elucidate mechanisms underlying bacterial virulence. For instance, EsaB has been hypothesized to act as a metallochaperone or to influence biofilm formation, though its exact molecular role remains under investigation.
Research on EsaB also extends to diagnostic and vaccine development. As a virulence-associated antigen, it holds potential as a biomarker for detecting *A. paragallinarum* infections or as a component of subunit vaccines to elicit protective immunity. Additionally, comparative genomics highlights *esaB* homologs in other pathogenic bacteria, suggesting conserved functions across species, which broadens its relevance in studying bacterial adaptation and evolution. Current challenges include resolving its 3D structure and clarifying its interaction networks, which are critical for translating basic research into applied solutions for combating bacterial diseases in agriculture and medicine.
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