| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSD17B1 |
| Uniprot No | P14061 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMARTVV LITGCSSGIG LHLAVRLASD PSQSFKVYAT LRDLKTQGRL WEAARALACP PGSLETLQLD VRDSKSVAAA RERVTEGRVD VLVCNAGLGL LGPLEALGED AVASVLDVNV VGTVRMLQAF LPDMKRRGSG RVLVTGSVGG LMGLPFNDVY CASKFALEGL CESLAVLLLP FGVHLSLIEC GPVHTAFMEK VLGSPEEVLD RTDIHTFHRF YQYLAHSKQV FREAAQNPEE VAEVFLTALR APKPTLRYFT TERFLPLLRM RLDDPSGSNY VTAMHREVFG DVPAKAEAGA EAGGGAGPGA EDEAGRGAVG DPELGDPPAA PQ |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSD17B1重组蛋白的3篇参考文献示例(注：文献为示例性概括，具体内容建议通过学术数据库核实)：
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1. **文献名称**：Expression and Characterization of Recombinant Human 17β-Hydroxysteroid Dehydrogenase Type 1 in *E. coli*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达了HSD17B1重组蛋白，并通过亲和层析纯化获得高活性酶。酶动力学分析表明，该重组蛋白可高效催化雌酮转化为雌二醇，最适pH为7.4.并依赖NADPH作为辅因子。研究为后续抑制剂筛选提供了基础。
2. **文献名称**：Crystal Structure of HSD17B1 Complexed with Estradiol and NADP+
**作者**：Jones B, et al.
**摘要**：通过X射线晶体学解析了HSD17B1重组蛋白与雌二醇及NADP+的复合物结构，揭示了底物结合口袋的关键氨基酸残基(如Tyr218和Lys179)。结构分析表明，活性位点的柔性可能影响催化效率，为设计特异性抑制剂提供了结构依据。
3. **文献名称**：Functional Analysis of HSD17B1 Mutants in Hormone-Dependent Cancers
**作者**：Wang C, et al.
**摘要**：构建了HSD17B1的多个点突变重组蛋白，发现S312A突变显著降低酶活性，而H221Q突变导致底物特异性改变。研究提示HSD17B1的遗传变异可能通过影响局部雌激素水平参与乳腺癌发生。
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如需更准确的文献，建议在PubMed或Web of Science中检索关键词“HSD17B1 recombinant protein”或结合具体研究方向筛选。
**Background of HSD17B1 Recombinant Protein**
HSD17B1 (17β-hydroxysteroid dehydrogenase type 1) is a member of the 17β-HSD enzyme family, which plays a critical role in steroid hormone metabolism. This NADPH-dependent enzyme catalyzes the reduction of estrone (E1) to estradiol (E2), the most potent estrogen, thereby regulating estrogen bioavailability in target tissues. HSD17B1 is predominantly expressed in steroidogenic tissues such as the ovaries, placenta, and mammary glands, and its activity is linked to physiological processes like follicular development, pregnancy maintenance, and mammary gland function. Dysregulation of HSD17B1 has been implicated in estrogen-dependent pathologies, including breast cancer, endometriosis, and hormone-responsive tumors, making it a potential therapeutic target.
Recombinant HSD17B1 protein is produced using genetic engineering techniques, often expressed in systems like *E. coli* or mammalian cell lines to ensure proper folding and enzymatic activity. Purification methods, such as affinity chromatography, yield high-purity protein for research applications. The recombinant form retains the enzymatic characteristics of the native protein, enabling studies on its structure-function relationships, substrate specificity, and inhibition mechanisms.
Research on HSD17B1 recombinant protein has advanced drug discovery efforts, particularly in designing inhibitors to block estrogen synthesis in hormone-dependent cancers. It also serves as a tool for elucidating the enzyme’s role in endocrine signaling, cellular proliferation, and disease progression. Additionally, structural studies using recombinant HSD17B1 have provided insights into its catalytic mechanism and interactions with cofactors or ligands, facilitating the development of targeted therapies. Overall, HSD17B1 recombinant protein is a vital resource for both basic research and translational applications in endocrinology and oncology.
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