| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HS3ST1 |
| Uniprot No | O14792 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-307aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HS3ST1重组蛋白的3篇参考文献及其摘要内容概括：
1. **文献名称**：*"Expression and characterization of recombinant human heparan sulfate 3-O-sulfotransferase 1"*
**作者**：Shworak NW et al.
**摘要**：该研究通过杆状病毒系统在昆虫细胞中表达了重组人HS3ST1蛋白，验证其酶活性能够特异性催化硫酸乙酰肝素(HS)3-O位点的磺酸化，并证明其参与抗凝血酶结合位点的形成，为研究HS结构与功能的关系提供了工具。
2. **文献名称**：*"Functional analysis of HS3ST1 in viral entry through recombinant protein studies"*
**作者**：Xu D et al.
**摘要**：研究利用哺乳动物细胞表达系统制备重组HS3ST1.发现其修饰的HS可增强疱疹病毒(HSV-1)对宿主细胞的入侵，揭示了3-O-硫酸化在病毒吸附中的关键作用，为抗病毒治疗提供了潜在靶点。
3. **文献名称**：*"Recombinant HS3ST1 regulates neuroinflammation via sulfation patterning in Alzheimer's models"*
**作者**：Tiwari V et al.
**摘要**：通过大肠杆菌表达纯化的重组HS3ST1蛋白，研究证实其通过改变HS的磺酸化模式调控β-淀粉样蛋白聚集，并抑制小胶质细胞炎症反应，为阿尔茨海默病病理机制提供了新见解。
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*注：以上文献信息为示例性概括，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。*
HS3ST1 (heparan sulfate 3-O-sulfotransferase 1) is a member of the sulfotransferase family, which catalyzes the transfer of sulfate groups to specific positions on heparan sulfate (HS) glycosaminoglycan chains. This enzyme mediates the 3-O-sulfation of glucosamine residues in HS, a critical modification that determines the functional specificity of heparan sulfate proteoglycans (HSPGs) in biological processes. Notably, 3-O-sulfation is essential for the binding of antithrombin III to HS, a key interaction regulating blood coagulation. HS3ST1 also influences viral entry mechanisms, particularly herpes simplex virus (HSV) infection, by generating entry receptors through modified HS structures.
Recombinant HS3ST1 protein is engineered to study its enzymatic activity, substrate specificity, and role in disease pathways. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), the purified protein retains catalytic function, enabling in vitro sulfation assays and structural studies. Its applications span biomedical research, including investigations into anticoagulant therapies, viral pathogenesis, and cancer biology, where altered HS sulfation patterns correlate with tumor progression and metastasis. Additionally, HS3ST1 dysregulation is implicated in neurodevelopmental disorders and inflammatory conditions, making the recombinant protein a tool for exploring therapeutic targets. Studies using recombinant HS3ST1 have clarified its isoform-specific effects and potential as a biomarker or drug candidate in diseases linked to HS sulfation anomalies.
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