| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HRS |
| Uniprot No | P12081 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-509aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAERAALEELVKLQGERVRGLKQQKAS AELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVF DVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSL RYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFD IAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVS DSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGV SLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARG LDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKV PCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKL VSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIK LRSVTSREEVDVRREDLVEEIKRRTGQPLCIC |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HRS(Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate)重组蛋白的参考文献示例，涵盖结构、功能及应用研究：
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1. **文献名称**：*Purification and Functional Characterization of Recombinant HRS Protein in the ESCRT-0 Complex*
**作者**：Smith A, et al.
**摘要**：本研究报道了重组HRS蛋白在大肠杆菌中的高效表达与纯化方法，并验证其作为ESCRT-0复合体核心组分的功能，证明其通过泛素化依赖的途径参与细胞膜蛋白分选及溶酶体降解过程。
2. **文献名称**：*Structural Insights into HRS Ubiquitin-Binding Domain via X-ray Crystallography*
**作者**：Zhang Y, et al.
**摘要**：通过X射线晶体学解析了HRS蛋白的泛素结合结构域(UIM)的三维结构，揭示了其与多聚泛素链相互作用的分子机制，为理解HRS在细胞内运输中的调控作用提供结构基础。
3. **文献名称**：*HRS Recombinant Protein as a Potential Therapeutic Target in Cancer Metastasis*
**作者**：Lee H, et al.
**摘要**：探讨了重组HRS蛋白在肿瘤细胞迁移中的作用，发现其过表达可抑制EGFR信号通路的内吞降解，提示靶向HRS可能成为抑制癌症转移的新策略。
4. **文献名称**：*Development of a HRS-Based Biosensor for Detecting Extracellular Vesicle Release*
**作者**：Johnson R, et al.
**摘要**：利用重组HRS蛋白对泛素化标记的特异性识别，构建了一种新型生物传感器，实时监测外泌体分泌过程，为研究细胞通讯提供技术工具。
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**注**：以上文献为示例性内容，实际引用时请通过学术数据库(如PubMed、Web of Science)检索最新且经同行评审的论文。
Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS), also known as HGS or HRS, is a multifunctional adaptor protein involved in intracellular trafficking, signal transduction, and membrane receptor sorting. It was initially identified as a substrate for tyrosine phosphorylation induced by hepatocyte growth factor (HGF). Structurally, HRS contains several functional domains, including a VHS (Vps27/Hrs/STAM) domain, FYVE (Fab1. YOTB, Vac1. EEA1) zinc finger domain, and ubiquitin-interacting motifs (UIMs), which enable its role in recognizing ubiquitinated cargo proteins and mediating endosomal sorting.
HRS plays a pivotal role in the endosomal sorting complex required for transport (ESCRT) pathway, facilitating the sorting of ubiquitinated membrane proteins into intraluminal vesicles of multivesicular bodies (MVBs). This process is critical for lysosomal degradation of receptors like EGFR, thereby regulating growth factor signaling and cellular homeostasis. Dysregulation of HRS is linked to cancer progression, neurodegenerative disorders, and immune dysfunctions due to impaired protein trafficking or aberrant signaling.
Recombinant HRS protein is typically produced using expression systems like Escherichia coli or mammalian cells, followed by affinity chromatography purification. Its recombinant form serves as a vital tool for studying endosomal dynamics, ubiquitin-dependent sorting mechanisms, and interactions with ESCRT components. Researchers also utilize it to investigate pathological mechanisms or screen therapeutic agents targeting HRS-related pathways. Additionally, engineered HRS variants help dissect domain-specific functions, offering insights into its regulatory roles in autophagy, viral budding, and exosome biogenesis. Its versatile involvement in cellular processes underscores its significance in both basic research and potential clinical applications.
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