| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | odh1 |
| Uniprot No | Q9BHM6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MTVKVCVCGGGNGAHTLSGLAASRDGVEVRVLTLFADEAERWTKALGADELTVIVNEKDGTQTEVKSRPKVITKDPEIAISGADVVILTVPAFAHEGYFQAMAPYVQDSALIVGLPSQAGFEFQCRDILGDKAAAVSMMSFETLPWACRIKEFGRKVEVLGTKSVLAASLIKGTAKTVDPLSTLQMLHGAEPVFRLAKHFLEMLIMSYSFVHPAILFGRWGSWDGKPVPEAPLFYQGIDQATADMLTACSNECKDVANAIMAACPGNDLSDVKDIYQWYLEYYHEDIQDDHDLYHAITTNKSYKGLVHPVKAVDGGVAPDFGNRYLTEDIPMGMIVFKGVAIAAGVAIPSNDKLIMWAQEKIGKEYLVDGALTGKDVATTRCPQRYGFNTLDAILTGKK |
| 预测分子量 | 50.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ODH1重组蛋白的参考文献示例(注：以下内容为模拟示例，非真实文献)：
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1. **文献名称**: *Heterologous Expression and Functional Characterization of ODH1 in Escherichia coli*
**作者**: Müller A, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组ODH1蛋白，并对其进行了纯化和酶动力学分析。结果显示ODH1在α-酮戊二酸脱羧反应中具有关键作用，为代谢工程改造提供了依据。
2. **文献名称**: *Structural Insights into ODH1 Catalytic Mechanism by X-ray Crystallography*
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析了酵母源ODH1的三维结构，揭示了其底物结合口袋和辅酶结合位点的构象变化，阐明了其催化循环的分子机制。
3. **文献名称**: *Role of Recombinant ODH1 in Plant Oxalate Metabolism*
**作者**: Chen L, et al.
**摘要**: 利用重组拟南芥ODH1蛋白，证实其在草酸分解代谢中的功能，过表达该酶显著增强植物对病原菌侵染的抗性，为作物抗病育种提供了新思路。
4. **文献名称**: *ODH1 Deficiency and Metabolic Reprogramming: Insights from Recombinant Protein Rescue Studies*
**作者**: Gupta R, et al.
**摘要**: 通过体外重组ODH1蛋白回补实验，证明其在三羧酸循环中的核心作用，并揭示了ODH1缺失导致线粒体功能障碍的分子机制。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“ODH1 recombinant protein”或结合具体物种名称(如“ODH1 Saccharomyces cerevisiae”)进一步筛选。
ODH1 (2-Oxoglutarate Dehydrogenase 1), a critical enzyme in the tricarboxylic acid (TCA) cycle, catalyzes the oxidative decarboxylation of 2-oxoglutarate (α-ketoglutarate) to succinyl-CoA, linking carbon metabolism with energy production and biosynthetic pathways. This mitochondrial matrix enzyme operates as part of a multi-enzyme complex requiring thiamine pyrophosphate (TPP), lipoic acid, and Coenzyme A as cofactors. Its activity is essential for cellular ATP generation, redox balance, and the synthesis of key metabolites, including amino acids and nucleotides. Dysregulation of ODH1 has been implicated in metabolic disorders, neurodegenerative diseases, and cancer, where altered TCA cycle flux influences tumor progression and metabolic reprogramming.
Recombinant ODH1 protein is produced using heterologous expression systems, such as *E. coli* or insect cells, to enable large-scale purification for functional and structural studies. Cloning the human or species-specific *odh1* gene into expression vectors allows controlled production, often tagged with affinity markers (e.g., His-tag) for simplified purification. Recombinant ODH1 retains enzymatic activity when reconstituted with cofactors, making it valuable for *in vitro* assays to study substrate specificity, inhibitor screening, or mutational effects on enzyme kinetics. It also serves as an antigen for antibody development or a tool to investigate mitochondrial dysfunction in disease models. Recent research leverages recombinant ODH1 to explore its non-metabolic roles, including epigenetic regulation and redox signaling, highlighting its multifaceted impact beyond central metabolism. This protein thus bridges basic biochemical inquiry with translational applications in drug discovery and metabolic disease therapeutics.
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