| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSD17B11 |
| Uniprot No | Q8NBQ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-285aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMESFVKLFIPKRRKSVTGEIVLITGAGHGI GRLTAYEFAKLKSKLVLWDINKHGLEETAAKCKGLGAKVHTFVVDCSNRE DIYSSAKKVKAEIGDVSILVNNAGVVYTSDLFATQDPQIEKTFEVNVLAH FWTTKAFLPAMTKNNHGHIVTVASAAGHVSVPFLLAYCSSKFAAVGFHKT LTDELAALQITGVKTTCLCPNFVNTGFIKNPSTSLGPTLEPEEVVNRLMH GILTEQKMIFIPSSIAFLTTLERILPERFLAVLKQKI |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSD17B11重组蛋白的模拟参考文献示例(注：部分信息为虚构，仅供格式参考)：
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1. **Title**: "Recombinant expression and functional characterization of human HSD17B11 in *E. coli*"
**Authors**: Smith, J.R., et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了带有His标签的HSD17B11重组蛋白，证实其对多种类固醇底物的17β-羟基脱氢酶活性，并发现其在胆汁酸代谢中的潜在作用。
2. **Title**: "Structural insights into HSD17B11: Crystallographic analysis of a steroid-metabolizing enzyme"
**Authors**: Lee, S., & Zhang, W.
**摘要**: 利用X射线晶体学解析了HSD17B11的3D结构，揭示了其底物结合口袋的特征，并发现其与家族其他成员的结构差异，为靶向药物设计提供了依据。
3. **Title**: "HSD17B11 overexpression promotes lipid accumulation in hepatocytes: Implications for metabolic syndrome"
**Authors**: Tanaka, K., et al.
**摘要**: 通过哺乳动物细胞重组表达HSD17B11.发现其过表达导致肝细胞脂质蓄积，提示该酶可能通过调节类固醇激素代谢参与代谢综合征的发展。
4. **Title**: "Development of a high-throughput assay for HSD17B11 enzymatic activity using recombinant protein"
**Authors**: Müller, F., et al.
**摘要**: 基于昆虫细胞表达的HSD17B11重组蛋白，建立了一种荧光底物检测法，用于高通量筛选其抑制剂，为治疗相关疾病提供了工具。
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**建议**：实际文献检索可通过PubMed、Google Scholar等平台，以“HSD17B11 recombinant protein”“HSD17B11 expression”等关键词查询。如需具体文献协助，请提供更多研究背景!
**Background of HSD17B11 Recombinant Protein**
HSD17B11 (17β-Hydroxysteroid Dehydrogenase Type 11) is a member of the 17β-hydroxysteroid dehydrogenase (17β-HSD) enzyme family, which plays a critical role in steroid hormone metabolism by catalyzing the oxidation or reduction of hydroxysteroids. This enzyme is implicated in the regulation of bioactive steroid levels, particularly androgens and estrogens, though its precise physiological substrates and functions remain under investigation. HSD17B11 is ubiquitously expressed, with notable presence in the liver, kidney, and reproductive tissues, suggesting involvement in systemic hormone homeostasis and metabolic pathways.
The recombinant HSD17B11 protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to enable high-purity, functional enzyme production for research. Its recombinant form allows detailed study of its enzymatic activity, substrate specificity, and interaction with cofactors like NAD+/NADH. Structural studies using recombinant HSD17B11 have revealed insights into its conserved short-chain dehydrogenase/reductase (SDR) fold and catalytic mechanisms.
Research links HSD17B11 to metabolic disorders, including obesity and fatty liver disease, as it may influence lipid metabolism and cellular stress responses. Additionally, its potential role in cancer progression, particularly hormone-sensitive cancers, is an emerging focus. Recombinant HSD17B11 serves as a vital tool for drug discovery, enabling screening of inhibitors or modulators targeting steroid-related pathologies. Despite progress, further studies are needed to fully elucidate its biological significance and therapeutic potential.
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