| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Mgat3 |
| Uniprot No | Q86VF5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-341aa |
| 氨基酸序列 | MGVATTLQPPTTSKTLQKQHLEAVGAYQYVLTFLFMGPFFSLLVFVLLFTSLWPFSVFYLVWLYVDWDTPNQGGRRSEWIRNRAIWRQLRDYYPVKLVKTAELPPDRNYVLGAHPHGIMCTGFLCNFSTESNGFSQLFPGLRPWLAVLAGLFYLPVYRDYIMSFGLCPVSRQSLDFILSQPQLGQAVVIMVGGAHEALYSVPGEHCLTLQKRKGFVRLALRHGASLVPVYSFGENDIFRLKAFATGSWQHWCQLTFKKLMGFSPCIFWGRGLFSATSWGLLPFAVPITTVVGRPIPVPQRLHPTEEEVNHYHALYMTALEQLFEEHKESCGVPASTCLTFI |
| 预测分子量 | 38.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Mgat3(N-乙酰葡萄糖胺转移酶III)重组蛋白的虚构参考文献示例(基于公开研究主题概括，非真实文献)：
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1. **文献名称**：*Recombinant MGAT3 Expression in HEK293 Cells Modulates IgG Glycosylation and Enhances Antibody-Dependent Cellular Cytotoxicity*
**作者**：Tanaka K, et al.
**摘要**：研究利用HEK293细胞表达重组MGAT3蛋白，证明其催化IgG的GlcNAc分支结构修饰，增强抗体Fc段的ADCC活性，为癌症免疫治疗提供新策略。
2. **文献名称**：*Functional Characterization of Recombinant MGAT3 in Glycoengineering of Therapeutic Proteins*
**作者**：Zhang Y, Wang L.
**摘要**：通过大肠杆菌系统表达可溶性重组MGAT3.验证其对促红细胞生成素(EPO)的糖基化改造能力，显著延长药物半衰期并提高稳定性。
3. **文献名称**：*MGAT3 Knockout and Recombinant Rescue in a Mouse Model Reveals Its Role in Neurodegenerative Disease*
**作者**：Chen H, et al.
**摘要**：通过重组MGAT3蛋白回补实验，发现其可恢复阿尔茨海默病模型小鼠中β-淀粉样蛋白异常糖基化水平，减缓神经元损伤。
4. **文献名称**：*Structural Analysis of Recombinant MGAT3 Using Cryo-EM and Its Substrate Specificity*
**作者**：Lee S, et al.
**摘要**：解析重组MGAT3的冷冻电镜结构，揭示其底物结合域的关键氨基酸残基，为设计小分子抑制剂调节糖基化提供结构基础。
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**注**：以上文献为基于MGAT3研究领域的合理虚构，实际文献需通过PubMed/Google Scholar检索关键词如“MGAT3 recombinant protein”或“GnT-III expression”获取。
Mgat3. also known as N-acetylglucosaminyltransferase III, is a key enzyme encoded by the MGAT3 gene in humans. It plays a critical role in N-glycosylation, a post-translational modification process that attaches complex carbohydrate structures (glycans) to proteins. Specifically, Mgat3 catalyzes the transfer of N-acetylglucosamine (GlcNAc) to the β-linked mannose residue of glycoproteins, forming a "bisecting GlcNAc" branch. This unique modification influences protein folding, stability, and cellular interactions by altering the overall structure of glycans.
Recombinant Mgat3 protein is engineered using genetic technology, often expressed in mammalian or insect cell systems to ensure proper glycosylation and functional activity. Researchers utilize this purified enzyme to study glycan biosynthesis, cellular signaling, and disease mechanisms. Its bisecting GlcNAc activity is particularly significant in cancer biology, as altered Mgat3 expression correlates with tumor progression, metastasis, and immune evasion. For example, reduced Mgat3 levels in certain cancers are linked to enhanced EGFR signaling and poor prognosis.
In therapeutic contexts, recombinant Mgat3 serves as a tool to modulate glycan structures on therapeutic antibodies, potentially improving their efficacy or reducing immunogenicity. It also aids in deciphering glycan-related pathways in autoimmune diseases and neurodegenerative disorders. Despite its specialized role, Mgat3's broad impact on cellular communication makes it a focal point for glycobiology research and biopharmaceutical development.
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