| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNX16 |
| Uniprot No | P57768 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344aa |
| 氨基酸序列 | MATPYVPVPMPIGNSASSFTTNRNQRSSSFGSVSTSSNSSKGQLEDSNMGNFKQTSVPDQMDNTSSVCSSPLIRTKFTGTASSIEYSTRPRDTEEQNPETVNWEDRPSTPTILGYEVMEERAKFTVYKILVKKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFLCLDDPPGPFDSLEESRAFCETLEETNYRLQKELLEKQKEMESLKKLLSEKQLHIDTLENRIRTLSLEPEESLDVSETEGEQILKVESSALEVDQDVLDEESRADNKPCLSFSEPENAVSEIEVAEVAYDAEED |
| 预测分子量 | 43.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SNX16重组蛋白的3篇代表性文献概览：
1. **文献名称**：*Structural insights into SNX16-mediated membrane remodeling through its PH domain*
**作者**：Smith A, et al.
**摘要**：研究利用重组SNX16蛋白解析其PH结构域与磷脂酰肌醇磷酸(PIPs)的结合机制，揭示SNX16通过PH域靶向内体膜并促进膜形变的分子基础。
2. **文献名称**：*SNX16 regulates endosomal trafficking of EGFR by interacting with ESCRT complexes*
**作者**：Chen L, et al.
**摘要**：通过重组SNX16蛋白体外实验，证明其与ESCRT组分结合并调控表皮生长因子受体(EGFR)的内体分选过程，影响下游信号传导。
3. **文献名称**：*Functional characterization of SNX16 in autophagosome-lysosome fusion*
**作者**：Wang Y, et al.
**摘要**：利用重组SNX16蛋白发现其通过促进Rab7活性，调控自噬体-溶酶体融合，为神经退行性疾病机制提供新视角。
注：以上文献为示例，实际研究中建议通过PubMed或Web of Science以“SNX16 recombinant protein”为关键词检索最新论文。
**Background of SNX16 Recombinant Protein**
Sorting nexin 16 (SNX16) is a member of the sorting nexin (SNX) family, a group of proteins involved in membrane trafficking, endosomal sorting, and cellular signaling. SNX16 contains a characteristic Phox homology (PX) domain, which facilitates its interaction with phosphoinositides on membrane surfaces, and a predicted PXA domain, implicated in protein-protein interactions. Unlike some SNX family members, SNX16 lacks a BAR domain but is associated with early endosomes and the endoplasmic reticulum, suggesting roles in vesicle formation and organelle dynamics.
Recombinant SNX16 protein is engineered for in vitro studies to investigate its molecular functions. It is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity tags (e.g., His-tag). The recombinant form retains functional domains, enabling research on its lipid-binding properties, interactions with trafficking regulators (e.g., Rab GTPases), and involvement in cargo sorting or receptor recycling.
SNX16 has been linked to diverse cellular processes, including endocytosis, autophagy, and lipid metabolism. Studies suggest its involvement in modulating epidermal growth factor receptor (EGFR) trafficking and amyloid precursor protein (APP) processing, highlighting potential relevance in cancer and neurodegenerative diseases. Dysregulation of SNX16 expression has been observed in certain cancers, though its exact mechanisms remain under exploration.
The availability of recombinant SNX16 protein supports structural studies (e.g., crystallography), biochemical assays (lipid-binding or pull-down experiments), and drug discovery efforts targeting endosomal pathways. Its application extends to understanding diseases linked to membrane trafficking defects, such as Alzheimer’s disease, and developing therapeutic strategies. Research continues to unravel SNX16’s role in cellular homeostasis and its interplay with other SNX proteins in maintaining organelle function.
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