| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3 |
| Entrez GeneID | 10643 |
| clone | 1B4D5 |
| WB Predicted band size | 63.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human IGF2BP3 (AA: 430-579) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于IGF2BP3抗体的3篇参考文献及其摘要概括：
1. **"IGF2BP3 as a prognostic biomarker in pancreatic ductal adenocarcinoma"**
- **作者**: Li D, et al.
- **摘要**: 研究通过免疫组化分析发现，IGF2BP3蛋白在胰腺癌组织中高表达，并与患者生存率降低显著相关，提示其作为预后标志物的潜在价值。
2. **"The role of IGF2BP3 in colorectal cancer progression and its detection using a novel monoclonal antibody"**
- **作者**: Wang Y, et al.
- **摘要**: 开发了一种特异性抗IGF2BP3的单克隆抗体，验证其在结直肠癌组织中的高表达，并证明其通过调控Wnt/β-catenin通路促进肿瘤侵袭。
3. **"IGF2BP3 expression correlates with aggressive phenotypes in melanoma: A tissue microarray study"**
- **作者**: Smith JL, et al.
- **摘要**: 利用组织芯片和IGF2BP3抗体进行检测，发现其表达与黑色素瘤的转移和耐药性相关，可能成为治疗靶点。
4. **"Validation of a commercial IGF2BP3 antibody for immunohistochemical application in neuroblastoma"**
- **作者**: Garcia-Rojas M, et al.
- **摘要**: 验证了某商业抗体在神经母细胞瘤中的特异性，证实IGF2BP3高表达与肿瘤分期和不良预后相关，支持其临床实用性。
这些研究均通过抗体实验验证了IGF2BP3在肿瘤中的生物学功能及临床意义。如需具体文献来源，可进一步提供PMID或DOI。
The insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3), also known as IMP3. is a member of the conserved IGF2BP family involved in post-transcriptional regulation of RNA. It binds to target mRNAs, influencing their stability, localization, and translation, particularly during embryonic development. In healthy adult tissues, IGF2BP3 expression is generally low or absent but is frequently reactivated in various cancers, including colorectal, ovarian, pancreatic, and lung carcinomas. Its overexpression is associated with aggressive tumor behavior, metastasis, and poor prognosis, making it a potential biomarker for cancer diagnosis and therapeutic targeting.
IGF2BP3 antibodies are essential tools for detecting and quantifying IGF2BP3 protein levels in research and clinical settings. These antibodies are widely used in techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence to study its expression patterns, subcellular localization, and role in oncogenesis. Commercially available IGF2BP3 antibodies are typically raised against specific epitopes of the human protein, with clones such as 69.1 and D9N6D being commonly utilized. Validation of antibody specificity is critical, as cross-reactivity with other IGF2BP family members (e.g., IMP1 or IMP2) may occur. Researchers often emphasize the importance of using well-characterized antibodies to ensure reproducibility in studies exploring IGF2BP3’s functional contributions to cancer progression, stem cell biology, or RNA metabolism.
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