Mouse Monoclonal CCNE1 Antibody

The CCNE1 antibody is a crucial tool in studying cyclin E1. a protein encoded by the CCNE1 gene that regulates cell cycle progression. Cyclin E1 binds to cyclin-dependent kinase 2 (CDK2) to form a complex essential for the G1/S phase transition, driving DNA replication initiation and cell division. Dysregulation of CCNE1. particularly overexpression, is linked to cancer development, including ovarian, breast, and gastric cancers, where it promotes genomic instability, uncontrolled proliferation, and therapy resistance. CCNE1 antibodies are widely used in research to detect and quantify cyclin E1 expression in tissues or cell lines via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help assess CCNE1's role in tumorigenesis, prognosis, and treatment response. Commercially available CCNE1 antibodies are typically validated for specificity, targeting epitopes within the protein's N-terminal or C-terminal regions. Recent studies also explore CCNE1 as a predictive biomarker for CDK inhibitor therapies or PARP inhibitors in cancers with homologous recombination deficiencies. However, challenges remain in standardizing detection methods due to post-translational modifications (e.g., phosphorylation) and rapid ubiquitin-mediated degradation of cyclin E1 during the cell cycle.