| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KRP; AAT7; MLCK; MLCK1; MMIHS; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083 |
| Entrez GeneID | 4638 |
| clone | 7F2B11 |
| WB Predicted band size | 210kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MYLK (AA:1375-1524) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MYLK抗体的3篇参考文献示例(内容为模拟概括，仅供参考)：
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1. **文献名称**：*MYLK Antibody-Based Detection of Endothelial Dysfunction in Acute Lung Injury*
**作者**：Garcia JG et al.
**摘要**：该研究通过Western blot和免疫组化技术，利用MYLK特异性抗体揭示了MYLK在血管内皮屏障破坏中的关键作用，为急性肺损伤的分子机制提供了实验依据(*Am J Respir Crit Care Med*, 2015)。
2. **文献名称**：*MYLK Expression in Colorectal Cancer Metastasis: An Immunohistochemical Study*
**作者**：Chen L et al.
**摘要**：研究采用MYLK抗体对结直肠癌组织进行免疫组化分析，发现MYLK高表达与肿瘤转移和患者预后不良显著相关，提示其作为潜在生物标志物的价值(*Oncogene*, 2018)。
3. **文献名称**：*MYLK Gene Variants and Aortic Aneurysm: Role of Antibody-Based Functional Assays*
**作者**：Wang X et al.
**摘要**：通过MYLK抗体进行免疫共沉淀和荧光染色，研究者发现特定MYLK突变体导致平滑肌细胞收缩异常，阐明了其在主动脉瘤发病中的机制(*Circulation Research*, 2020)。
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**说明**：
- 以上文献为示例，实际引用需根据真实研究调整。
- 研究多聚焦于MYLK在疾病(如癌症、心血管疾病)中的功能及抗体应用(Western blot、免疫组化等)。
- 可通过PubMed或Google Scholar以“MYLK antibody”、“MYLK kinase immunohistochemistry”等关键词检索真实文献。
MYLK (myosin light-chain kinase) antibodies are immunological tools used to study the MYLK protein, a calcium/calmodulin-dependent enzyme critical for regulating smooth muscle and non-muscle cell contraction. MYLK phosphorylates the regulatory light chain of myosin, enabling actin-myosin interaction and cellular contraction. It exists in multiple isoforms, including the long (endothelial) and short (smooth muscle) forms, with tissue-specific expression patterns. MYLK plays roles in vascular tone, airway hyperreactivity, and endothelial barrier function, making it relevant to cardiovascular diseases, asthma, and sepsis.
Antibodies targeting MYLK are utilized in research to investigate its expression, localization, and function in physiological and pathological contexts. They are employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study MYLK's involvement in signaling pathways, such as TGF-β-mediated fibrosis or inflammation. Dysregulation of MYLK activity is linked to diseases like hypertension, acute lung injury, and cancer metastasis, where aberrant cell motility or vascular permeability occurs.
MYLK antibodies also aid in distinguishing between isoforms, helping clarify their distinct roles. For example, endothelial MYLK is implicated in vascular leakage, while smooth muscle MYLK regulates bronchoconstriction. Recent studies explore MYLK's potential as a therapeutic target, driving demand for specific antibodies to validate experimental models or biomarker discovery. However, challenges remain in ensuring isoform specificity and minimizing cross-reactivity in antibody validation.
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