| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGPAT4 |
| Uniprot No | Q9NRZ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-378aa |
| 氨基酸序列 | MDLAGLLKSQFLCHLVFCYVFIASGLIINTIQLFTLLLWPINKQLFRKINCRLSYCISSQLVMLLEWWSGTECTIFTDPRAYLKYGKENAIVVLNHKFEIDFLCGWSLSERFGLLGGSKVLAKKELAYVPIIGWMWYFTEMVFCSRKWEQDRKTVATSLQHLRDYPEKYFFLIHCEGTRFTEKKHEISMQVARAKGLPRLKHHLLPRTKGFAITVRSLRNVVSAVYDCTLNFRNNENPTLLGVLNGKKYHADLYVRRIPLEDIPEDDDECSAWLHKLYQEKDAFQEEYYRTGTFPETPMVPPRRPWTLVNWLFWASLVLYPFFQFLVSMIRSGSSLTLASFILVFFVASVGVRWMIGVTEIDKGSAYGNSDSKQKLND |
| 预测分子量 | 45.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于AGPAT4重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
1. **《Functional Characterization of Recombinant AGPAT4 in Lipid Biosynthesis》**
- 作者：Smith A, et al.
- 摘要：本研究在大肠杆菌中成功表达并纯化了AGPAT4重组蛋白，证实其在体外催化溶血磷脂酸(LPA)转化为磷脂酸(PA)的酶活性，揭示了其在甘油三酯合成中的关键作用。
2. **《AGPAT4 Overexpression Modulates Cellular Lipid Metabolism in Hepatocytes》**
- 作者：Wang X, et al.
- 摘要：通过哺乳动物细胞系统重组表达AGPAT4.发现其过表达显著增加肝细胞中脂滴形成，提示AGPAT4可能在非酒精性脂肪肝疾病(NAFLD)中发挥调控作用。
3. **《Structural Insights into AGPAT4 Catalytic Mechanism by Cryo-EM》**
- 作者：Li J, et al.
- 摘要：利用冷冻电镜解析了重组AGPAT4蛋白的三维结构，揭示了其酰基转移活性位点的关键氨基酸残基，为设计特异性抑制剂提供了结构基础。
4. **《AGPAT4 Knockdown Alters Cancer Cell Membrane Phospholipid Composition》**
- 作者：Garcia R, et al.
- 摘要：在乳腺癌细胞中敲低AGPAT4基因后，重组蛋白功能缺失导致细胞膜磷脂比例异常，抑制肿瘤细胞增殖和迁移，表明其潜在作为癌症治疗靶点。
**注意**：以上内容为模拟生成，如需真实文献，建议通过PubMed、Google Scholar或Web of Science等平台检索关键词“AGPAT4 recombinant protein”或“AGPAT4 lysophosphatidic acid acyltransferase”。
AGPAT4 (1-acylglycerol-3-phosphate O-acyltransferase 4) is a key enzyme in the biosynthesis of glycerophospholipids, which are essential components of cellular membranes. Belonging to the AGPAT family, it catalyzes the acylation of lysophosphatidic acid (LPA) to produce phosphatidic acid (PA), a central precursor for triglycerides and phospholipids. This reaction occurs at the sn-2 position of the glycerol backbone, influencing membrane fluidity, signaling, and lipid storage. AGPAT4 exhibits tissue-specific expression, with higher levels observed in the brain, liver, and adipose tissue, suggesting roles in lipid metabolism and neurological functions.
Recombinant AGPAT4 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies. The protein is typically purified via affinity tags (e.g., His-tag) for high yield and purity. Researchers utilize this tool to investigate enzymatic kinetics, substrate specificity, and regulatory mechanisms, particularly its preference for unsaturated fatty acyl-CoA donors. Dysregulation of AGPAT4 has been implicated in metabolic disorders, insulin resistance, and cancer progression, making it a potential therapeutic target. Recombinant AGPAT4 also aids in screening inhibitors or modulators for drug development. Its structural characterization, including conserved acyltransferase domains, provides insights into catalytic sites and mutation-related pathologies. Studies leveraging recombinant AGPAT4 contribute to understanding lipid homeostasis and developing interventions for lipid-associated diseases.
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