| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | POMC; LPH; MSH; NPP; POC; CLIP |
| Entrez GeneID | 5443 |
| clone | 3A4H4 |
| WB Predicted band size | 29.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ACTH expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于ACTH抗体的参考文献示例(信息基于公开文献数据，非实时更新)：
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1. **文献名称**：*Development of a Highly Specific Monoclonal Antibody for ACTH Immunoassays*
**作者**：Smith A, et al.
**摘要**：研究团队开发了一种针对ACTH的单克隆抗体，通过表位定位技术提高抗体特异性，验证其在血浆ACTH检测中的灵敏度和抗干扰能力，适用于库欣综合征的诊断。
2. **文献名称**：*ACTH Antibody Cross-reactivity in Commercial Immunoassays: A Comparative Study*
**作者**：Brown C, et al.
**摘要**：比较不同商业ACTH检测试剂盒中抗体的交叉反应性，发现部分抗体与α-MSH或CLIP等肽段存在交叉反应，提示临床诊断中需谨慎选择检测方法。
3. **文献名称**：*ACTH Measurement in Human Plasma: Validation of a Novel Chemiluminescent Assay*
**作者**：Zhang L, et al.
**摘要**：报道一种新型化学发光法检测ACTH的临床验证研究，使用高亲和力多克隆抗体，显著降低检测下限(LLoQ)，提升对低浓度ACTH样本的检出率。
4. **文献名称**：*Role of ACTH Antibody in the Pathophysiology of Autoimmune Hypophysitis*
**作者**：Tanaka K, et al.
**摘要**：探讨ACTH自身抗体在自身免疫性垂体炎中的作用，发现部分患者血清中存在针对ACTH的IgG抗体，可能与垂体功能减退相关。
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注：以上为模拟文献，实际引用时需通过PubMed、Web of Science等数据库检索真实论文并核对原文。
**Background of ACTH Antibodies**
Adrenocorticotropic hormone (ACTH), a 39-amino acid peptide hormone produced by the anterior pituitary gland, regulates cortisol synthesis in the adrenal cortex via the hypothalamic-pituitary-adrenal (HPA) axis. ACTH antibodies are immunochemical tools designed to detect and quantify ACTH in biological samples. These antibodies are critical in diagnosing endocrine disorders like Cushing’s syndrome, Addison’s disease, or pituitary tumors, where ACTH levels are dysregulated.
ACTH antibodies are typically generated by immunizing host animals (e.g., rabbits, mice) with synthetic ACTH fragments or recombinant proteins. Monoclonal antibodies target specific epitopes, ensuring high specificity, while polyclonal antibodies recognize multiple epitopes, enhancing sensitivity. Validation steps, including ELISA, immunohistochemistry, or Western blot, confirm their binding affinity and cross-reactivity profiles.
In research, ACTH antibodies help study HPA axis dynamics, stress responses, and glucocorticoid regulation. Clinically, they enable precise ACTH measurement in immunoassays, aiding differential diagnosis. Challenges include minimizing cross-reactivity with pro-opiomelanocortin (POMC) derivatives or species-specific ACTH variants. Advances in recombinant antibody technology and epitope mapping continue to improve their reliability for diagnostic and experimental applications.
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