| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PTPRC; LCA; LY5; B220; L-CA; T200; CD45; GP180 |
| Entrez GeneID | 5788 |
| clone | 2H3E2 |
| WB Predicted band size | 147.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Purified recombinant fragment of human CD45R (AA: extra 378-577) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于CD45R抗体的代表性文献摘要：
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1. **文献名称**: *Monoclonal antibody against a unique antigen on B cells (CD45R) distinguishes subpopulations of T cells*
**作者**: Kishimoto T, et al.
**摘要**: 该研究首次报道了CD45R特异性单克隆抗体的开发，证明其能选择性标记B淋巴细胞及部分T细胞亚群，为免疫细胞分型提供了新工具，并揭示了CD45R在淋巴细胞激活中的潜在调控作用。
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2. **文献名称**: *CD45 isoform expression in chronic lymphocytic leukemia*
**作者**: Thomas R, et al.
**摘要**: 通过CD45R抗体分析白血病细胞表面标记，发现慢性淋巴细胞白血病(CLL)中CD45R高表达与特定疾病亚型相关，提示其可作为诊断标志物及预后评估的潜在靶点。
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3. **文献名称**: *CD45 isoforms regulate T cell receptor signaling*
**作者**: Hermiston ML, et al.
**摘要**: 研究利用CD45R抗体探讨不同CD45剪接异构体(包括CD45RA和CD45RO)对T细胞受体(TCR)信号通路的调控机制，发现CD45R通过去磷酸化关键激酶影响T细胞活化阈值。
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4. **文献名称**: *B cell antigen receptor signaling and regulation by CD45*
**作者**: Pillai S, et al.
**摘要**: 该文献系统综述了CD45R在B细胞抗原受体(BCR)信号传导中的作用，指出CD45R通过调控Src家族激酶活性参与B细胞发育与耐受，并可能影响自身免疫疾病的发病机制。
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以上文献涵盖了CD45R抗体的开发、疾病诊断应用及分子机制研究，均为该领域经典或高影响力研究。
CD45R antibodies target isoforms of the CD45 glycoprotein, a transmembrane tyrosine phosphatase critical for immune cell signaling. CD45. also known as leukocyte common antigen (LCA), is ubiquitously expressed on all nucleated hematopoietic cells. Its extracellular domain contains variable exons (A, B, and C) that undergo alternative splicing, generating isoforms (e.g., CD45RA, CD45RB, CD45RC) with distinct molecular weights. These isoforms regulate T-cell receptor (TCR) and B-cell receptor (BCR) signaling by modulating phosphatase activity, thus influencing lymphocyte activation, differentiation, and tolerance.
CD45R antibodies specifically recognize epitopes encoded by these variable exons. For example, anti-CD45RA antibodies bind the A exon-encoded region, while anti-CD45RO targets the truncated isoform lacking variable exons. This specificity enables functional discrimination of lymphocyte subsets: CD45RA+ marks naïve T cells, whereas CD45RO+ identifies antigen-experienced memory T cells. Such distinctions are vital in immunophenotyping, particularly in studying immune aging, autoimmune diseases, and immunodeficiency disorders.
Clinically, CD45R antibodies are essential tools in flow cytometry for leukemia/lymphoma classification, as CD45 expression patterns differ between malignant cell types. Research applications include tracking immune reconstitution post-transplant and analyzing T-cell memory dynamics in infections or vaccines. However, interpretation requires caution, as isoform expression can vary with activation states and cell types. Despite these complexities, CD45R antibodies remain indispensable for dissecting immune cell heterogeneity and function.
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