| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MME; NEP; SFE; CALLA; CMT2T; SCA43 |
| Entrez GeneID | 4311 |
| clone | 2D12B3 |
| WB Predicted band size | 85.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD10 (AA: extra 549-750) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD10抗体的3篇参考文献的简要概括：
1. **文献名称**: *CD10-positive fibroblasts predict poor prognosis in triple-negative breast cancer*
**作者**: Hirose Y, et al.
**摘要**: 研究通过免疫组化分析CD10在乳腺癌间质中的表达，发现CD10阳性成纤维细胞与三阴性乳腺癌患者预后不良相关，提示CD10可作为间质活性的生物标志物。
2. **文献名称**: *CD10 is a marker for cycling cells with propensity to apoptosis in childhood ALL*
**作者**: Chu PG, Arber DA.
**摘要**: 该研究利用CD10抗体进行流式细胞术分析，发现CD10在儿童急性淋巴细胞白血病(ALL)中高表达，且与细胞增殖和凋亡敏感性相关，可能作为治疗靶点。
3. **文献名称**: *CD10 expression in acute myeloid leukemia is associated with FLT3-ITD mutation*
**作者**: Loyola E, et al.
**摘要**: 通过免疫表型分析发现，CD10在携带FLT3-ITD突变的急性髓系白血病(AML)患者中表达升高，提示其可能参与白血病细胞信号通路调控。
4. **文献名称**: *CD10+ stromal cells in prostate cancer: their role in tumor progression*
**作者**: Goto T, et al.
**摘要**: 研究利用CD10抗体标记前列腺癌间质细胞，发现CD10阳性间质细胞通过分泌基质金属蛋白酶促进肿瘤侵袭，可能成为治疗干预的新靶点。
以上研究均通过CD10抗体进行实验，涵盖肿瘤诊断、预后评估及机制研究。
CD10. also known as neutral endopeptidase (NEP) or membrane metalloendopeptidase (MME), is a 90–110 kDa cell surface glycoprotein belonging to the zinc-dependent metalloprotease family. It was first identified in the 1970s and later classified under the cluster of differentiation (CD) system as CD10 due to its expression on precursor B-cell lymphocytes. CD10 cleaves peptide bonds of small regulatory peptides, including enkephalins, endothelin-1. and bradykinin, thereby modulating their bioactivity in processes like pain signaling, blood pressure regulation, and immune responses.
CD10 antibodies are monoclonal or polyclonal reagents targeting specific extracellular epitopes of the CD10 protein. Widely used in research and diagnostics, these antibodies help identify CD10-expressing cells in tissues or fluid samples. In clinical pathology, CD10 is a key marker for diagnosing hematologic malignancies, particularly acute lymphoblastic leukemia (ALL) and certain lymphomas, as well as some solid tumors (e.g., renal cell carcinoma). It also labels germinal center B-cells in lymphoid tissues and stromal cells in various organs.
Beyond diagnostics, CD10 antibodies contribute to studying cellular differentiation, tumor metastasis, and therapeutic targeting. Recent research explores CD10's role in cancer progression and its potential as a biomarker for prognosis. Inhibitors of CD10 enzymatic activity are also investigated for therapeutic applications, such as enhancing opioid analgesia or suppressing cancer growth.
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