| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | gtfC |
| Uniprot No | P13470 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 426-597aa |
| 氨基酸序列 | TIGGYEFLLANDVDNSNPVVQAEQLNWLHFLMNFGNIYANDPDANFDSIRVDAVDNVDADLLQIAGDYLKAAKGIHKNDKAANDHLSILEAWSYNDTPYLHDDGDNMINMDNRLRLSLLYSLAKPLNQRSGMNPLITNSLVNRTDDNAETAAVPSYSFIRAHDSEVQDLIRN |
| 预测分子量 | 26.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟的关于 **gtfC重组蛋白** 的参考文献示例(实际文献需通过学术数据库检索)：
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1. **文献名称**: *"Heterologous Expression and Functional Characterization of Recombinant GtfC from Streptococcus mutans in Escherichia coli"*
**作者**: Smith A, et al.
**摘要**: 本研究成功克隆了变形链球菌(*S. mutans*)的 *gtfC* 基因，并在大肠杆菌中实现重组表达。纯化后的重组GtfC蛋白在体外表现出葡聚糖合成活性，为研究其在生物膜形成中的作用提供了工具。
2. **文献名称**: *"Structural Insights into the Catalytic Mechanism of Recombinant GtfC Glucosyltransferase"*
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析了重组GtfC蛋白的三维结构，揭示了其底物结合域和催化活性位点的关键残基，阐明了其在水不溶性葡聚糖合成中的分子机制。
3. **文献名称**: *"Immunogenicity of Recombinant GtfC Protein as a Potential Anti-Caries Vaccine Antigen"*
**作者**: Chen L, et al.
**摘要**: 评估了重组GtfC蛋白在小鼠模型中的免疫原性，结果显示其可诱导特异性抗体产生，显著抑制变形链球菌在牙齿表面的定植，提示其在龋齿疫苗开发中的潜力。
4. **文献名称**: *"Optimization of Recombinant GtfC Production in Pichia pastoris for Large-Scale Applications"*
**作者**: Müller R, et al.
**摘要**: 比较了毕赤酵母与大肠杆菌表达系统对重组GtfC蛋白产量的影响，通过发酵条件优化使蛋白产量提高3倍，为工业级酶生产提供了参考。
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**备注**：以上文献为模拟示例，实际研究需通过 **PubMed**、**Web of Science** 或 **Google Scholar** 检索关键词：
`"gtfC recombinant protein"`, `"glucosyltransferase C expression"`, `"Streptococcus mutans gtfC"`。
**Background of gtfC Recombinant Protein**
The **gtfC gene** encodes a glucosyltransferase (GTF) enzyme, primarily produced by *Streptococcus mutans*, a key pathogen associated with dental caries. This enzyme catalyzes the synthesis of water-insoluble glucans from sucrose, facilitating bacterial adhesion to tooth surfaces and promoting biofilm formation. As a virulence factor, GtfC contributes to the pathogenesis of dental plaque and tooth decay by enhancing microbial colonization and acid production within oral biofilms.
Recombinant gtfC protein is generated through genetic engineering, where the *gtfC* gene is cloned into expression vectors (e.g., *E. coli* or yeast systems) to produce purified protein for research or therapeutic applications. Its recombinant form allows for controlled production, enabling studies on enzyme kinetics, structure-function relationships, and interactions with inhibitors. Researchers utilize gtfC recombinant protein to develop anti-caries strategies, such as designing vaccines or small-molecule inhibitors targeting GTF activity to disrupt biofilm formation.
Additionally, gtfC has been explored in structural biology to resolve its 3D conformation, revealing critical domains like the catalytic site and glucan-binding regions. These insights aid in rational drug design. Beyond dental research, recombinant gtfC serves as an antigen in immunoassays to detect *S. mutans* antibodies in clinical samples, supporting caries risk assessment.
Overall, gtfC recombinant protein is a vital tool for understanding microbial pathogenesis, advancing anti-biofilm therapies, and improving diagnostic approaches in oral health. Its study bridges microbiology, enzymology, and translational dentistry, highlighting its significance in both basic and applied research.
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