| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IER3 |
| Uniprot No | P46695 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-82aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMCHSRSCHPTMTILQAPTPAPSTIPGP RRGSGPEIFTFDPLPEPAAAPAGRPSASRGHRKRSRRVLYPRVVRRQLPV EEPNP |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IER3重组蛋白的3篇参考文献示例(注：文献为模拟示例，实际引用请核实真实来源)：
1. **《Recombinant human IER3 protein induces apoptosis in cancer cells via p53-dependent pathway》**
- 作者：Chen L, et al.
- 摘要：研究报道了在大肠杆菌中成功表达并纯化重组人IER3蛋白，体外实验表明该蛋白通过激活p53信号通路诱导肿瘤细胞凋亡，为癌症治疗提供潜在策略。
2. **《Characterization of IEX-1 recombinant protein in modulating inflammatory response》**
- 作者：Kim S, et al.
- 摘要：通过哺乳动物表达系统获得重组IEX-1(IER3)蛋白，发现其通过抑制NF-κB活性减少促炎因子分泌，提示其在炎症性疾病中的调控作用。
3. **《Structural and functional analysis of recombinant IER3 in stress-induced cellular senescence》**
- 作者：Wang Y, et al.
- 摘要：利用杆状病毒系统表达IER3重组蛋白，解析其与JNK激酶的相互作用机制，揭示其在氧化应激诱导的细胞衰老中的保护功能。
注：以上文献信息为学术场景模拟，实际文献需通过PubMed、Web of Science等平台检索确认。
**Background of IER3 Recombinant Protein**
The **Immediate Early Response 3 (IER3)**, also known as **IEX-1** or **Gly96**, is a stress-inducible protein encoded by the *IER3* gene. It belongs to the immediate-early gene family, which is rapidly activated in response to diverse cellular stimuli, including growth factors, cytokines, radiation, and oxidative stress. IER3 plays a dual role in regulating cell survival and apoptosis, depending on the cellular context and microenvironment. It interacts with key signaling pathways such as NF-κB, MAPK, and PI3K/AKT, modulating processes like proliferation, differentiation, and immune responses. Dysregulation of IER3 has been implicated in various diseases, including cancer, autoimmune disorders, and diabetic complications, highlighting its importance in cellular homeostasis and pathology.
Recombinant IER3 protein is produced using *in vitro* expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and bioactivity. This engineered protein retains the functional domains of native IER3. enabling researchers to study its molecular mechanisms, protein-protein interactions, and therapeutic potential. Applications include elucidating its role in stress responses, screening for inhibitors/activators in drug discovery, and developing diagnostic tools. The recombinant form also facilitates structural studies, epitope mapping for antibody development, and validation of cellular pathways in experimental models.
Overall, IER3 recombinant protein serves as a critical tool for unraveling the complex biological functions of IER3 and exploring its translational relevance in human health and disease.
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