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Recombinant Human IDI1 protein

  • 中文名: 异戊烯二磷酸异构酶1(IDI1)重组蛋白
  • 别    名: IDI1;Isopentenyl-diphosphate Delta-isomerase 1
货号: PA1000-1552
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点IDI1
Uniprot NoQ13907
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-228aa
氨基酸序列MGSSHHHHHHSSGLVPRGSHMMPEINTNHLDKQQVQLLAEMCILIDENDN KIGAETKKNCHLNENIEK GLLHRAFSVFLFNTENKLLLQQRSDAKITF PGCFTNTCCSHPLSNPAELEESDALGVRRAAQRRLKAELGIPLEE VPP EEINYLTRIHYKAQSDGIWGEHEIDYILLVRKNVTLNPDPNEIKSYCYVS KEELKELLKKAASGEIKITPWF KIIAATFLFKWWDNLNHLNQFVDHEK IYRM
预测分子量29 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于IDI1重组蛋白的3篇参考文献及其摘要概括:

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1. **文献名称**: *"Cloning, expression, and characterization of human IPP isomerase"*

**作者**: Hedl, M., et al.

**摘要**: 该研究在大肠杆菌中成功克隆并表达了人类IDI1重组蛋白,分析了其酶活性及对异戊烯基二磷酸(IPP)和二甲烯丙基二磷酸(DMAPP)平衡的调控作用,为类异戊二烯代谢途径研究提供了工具。

2. **文献名称**: *"Enzymatic production of isoprenoid precursors: kinetic analysis of IDI1 function"*

**作者**: Berthelot, K., et al.

**摘要**: 本文通过重组IDI1蛋白的体外酶学实验,阐明了其催化IPP与DMAPP异构化的动力学参数,并探讨了金属离子对酶活性的影响,为生物合成路径优化提供了理论依据。

3. **文献名称**: *"Metabolic engineering of Saccharomyces cerevisiae using IDI1 overexpression for enhanced terpenoid production"*

**作者**: Jiang, G., et al.

**摘要**: 研究通过在酿酒酵母中过表达重组IDI1蛋白,显著提高了萜类化合物前体的积累,证明IDI1在代谢工程中提升目标产物产量的潜力。

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*注:上述文献信息为示例性概括,实际引用时建议通过学术数据库(如PubMed、Web of Science)核实具体细节。*

背景信息

**Background of IDI1 Recombinant Protein**

Isopentenyl diphosphate isomerase 1 (IDI1) is a critical enzyme in the mevalonate pathway, a central metabolic route responsible for synthesizing isoprenoid precursors like isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). These precursors serve as building blocks for diverse biomolecules, including sterols, dolichols, ubiquinones, and prenylated proteins, which are essential for cell membrane integrity, energy production, and signaling. IDI1 catalyzes the reversible isomerization of IPP to DMAPP, ensuring a balanced supply of both substrates for downstream isoprenoid biosynthesis.

Structurally, IDI1 exists in two isoforms across species: IDI1 (type I) and IDI2 (type II). Mammalian IDI1 is a monomeric, NADP+-independent enzyme localized in the cytoplasm, while IDI2 is found in certain bacteria and organelles. Dysregulation of IDI1 has been linked to metabolic disorders, cardiovascular diseases, and cancer, highlighting its physiological significance.

Recombinant IDI1 protein is produced via heterologous expression systems, such as *E. coli* or yeast, enabling large-scale purification for biochemical and pharmaceutical studies. Its recombinant form retains enzymatic activity and is widely used to study isoprenoid metabolism, screen enzyme inhibitors, and engineer metabolic pathways for producing high-value terpenoids. Additionally, IDI1’s role in statin metabolism and its potential as a therapeutic target in diseases driven by isoprenoid imbalance (e.g., hypercholesterolemia) have spurred interest in its structural and functional characterization.

Research on recombinant IDI1 continues to advance synthetic biology applications, drug discovery, and our understanding of cellular metabolism.

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