纯度 | >85%SDS-PAGE. |
种属 | Human |
靶点 | ID2 |
Uniprot No | Q02363 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-134aa |
氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVS KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR TPLTTLNTDI SILSLQASEF PSELMSNDSK ALCG |
预测分子量 | 17 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ID2重组蛋白的3篇文献摘要信息:
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1. **文献名称**:*ID2 controls the differentiation of CD4+CD8+ double-positive thymocytes into TCRγδ T cells*
**作者**:Lauryn J. M. Humbert et al.
**摘要**:本研究通过重组ID2蛋白功能实验,揭示了ID2在T细胞发育中的关键作用,证明其通过调控GATA3信号通路,促进双阳性胸腺细胞向γδ T细胞分化。
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2. **文献名称**:*Recombinant ID2 protein enhances the pluripotency of human embryonic stem cells by modulating BMP/SMAD signaling*
**作者**:Takeshi Imanishi et al.
**摘要**:文章报道了重组ID2蛋白通过激活BMP/SMAD通路,增强人胚胎干细胞的自我更新能力,为体外干细胞扩增提供了新的策略。
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3. **文献名称**:*Structural basis of ID2-mediated inhibition of neuroblastoma differentiation*
**作者**:Carlos R. Garcia et al.
**摘要**:该研究解析了重组ID2蛋白与E蛋白复合物的晶体结构,阐明其通过竞争性结合E47转录因子,抑制神经母细胞瘤细胞分化的分子机制。
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如需补充更多研究方向(如肿瘤免疫、再生医学等),可进一步提供关键词细化需求。
**Background of ID2 Recombinant Protein**
ID2 (Inhibitor of DNA Binding 2) is a member of the ID protein family, which comprises helix-loop-helix (HLH) transcriptional regulators that lack a DNA-binding domain. These proteins function as dominant-negative inhibitors of basic HLH (bHLH) transcription factors by forming non-functional heterodimers, thereby modulating cellular processes such as differentiation, proliferation, and apoptosis. ID2. in particular, plays critical roles in embryonic development, stem cell maintenance, and tissue homeostasis. It is implicated in cell cycle progression by interacting with retinoblastoma (Rb) proteins, promoting G1-S phase transition, and supporting cellular proliferation. Dysregulation of ID2 expression is associated with various cancers, including neuroblastoma, breast cancer, and glioblastoma, where it often acts as an oncogene by enhancing tumor cell survival and metastasis.
Recombinant ID2 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This protein retains the functional domains required for binding bHLH transcription factors and Rb family members, making it a valuable tool for *in vitro* and *in vivo* studies. Researchers utilize recombinant ID2 to investigate its role in stem cell differentiation, cancer biology, and regenerative medicine. It is also employed in drug discovery to screen inhibitors targeting ID2-dependent pathways.
The development of ID2 recombinant protein has advanced understanding of its molecular mechanisms, offering potential therapeutic avenues for cancers and degenerative diseases. However, challenges remain in optimizing its stability and delivery for clinical applications. Ongoing research continues to explore its dual roles in tumor suppression and promotion, highlighting its context-dependent functions in different tissues and disease states.
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