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Recombinant Human IDH3G protein

  • 中文名: 异柠檬酸脱氢酶3(IDH3G)重组蛋白
  • 别    名: IDH3G;Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
货号: PA1000-1549
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点IDH3G
Uniprot NoP51553
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-393aa
氨基酸序列MALKVATVAGSAAKAVLGPALLCRPWEVLGAHEVPSRNIFSEQTIPPSAK YGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI RNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKS LPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRI AEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFEN MIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYG HVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAASIRK AVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIRVINGRAVEA
预测分子量69 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于IDH3G重组蛋白的模拟参考文献示例(请注意,文献信息为虚构,仅供格式参考):

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1. **"Recombinant Expression and Functional Characterization of Human IDH3G in Metabolic Regulation"**

*作者:Zhang Y, et al. (2020)*

**摘要**:本研究成功在大肠杆菌中表达并纯化了重组人源IDH3G蛋白,分析了其与IDH3α/β亚基的相互作用。实验表明IDH3G的磷酸化修饰显著影响异柠檬酸脱氢酶复合体的活性,提示其在三羧酸循环动态调控中的作用。

2. **"Structural Insights into IDH3G Mutations Associated with Mitochondrial Dysfunction"**

*作者:Chen L, et al. (2018)*

**摘要**:通过X射线晶体学解析了重组IDH3G蛋白的晶体结构,发现其Arg125His突变会导致与IDH3α亚基的结合能力下降,进而降低酶活性。该突变与线粒体能量代谢缺陷疾病相关。

3. **"IDH3G Deficiency Alters Cellular Redox Balance via Recombinant Protein-Based Assays"**

*作者:Wang T, et al. (2021)*

**摘要**:利用重组IDH3G蛋白构建体外酶活体系,发现IDH3G缺失会导致NADH生成减少和活性氧(ROS)积累,揭示了其在维持细胞氧化还原稳态中的关键作用。

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**建议**:实际研究中可通过PubMed、Web of Science等平台,以关键词“IDH3G recombinant protein”“IDH3 structure/function”等检索最新文献,重点关注《Nature Metabolism》《Cell Reports》等期刊。

背景信息

**Background of IDH3G Recombinant Protein**

Isocitrate dehydrogenase 3 gamma (IDH3G) is a subunit of the IDH3 enzyme, a critical component of the tricarboxylic acid (TCA) cycle responsible for catalyzing the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG), coupled with NADH production. IDH3. unlike its isoforms IDH1 and IDH2. functions exclusively in mitochondria and requires heterocomplex assembly with alpha (IDH3A) and beta (IDH3B) subunits for enzymatic activity. The IDH3G subunit plays a structural and regulatory role in stabilizing the enzyme complex and modulating its activity.

Recombinant IDH3G protein is engineered using biotechnological platforms (e.g., bacterial or mammalian expression systems) to produce purified, functional protein for research and therapeutic applications. Its production enables detailed studies of IDH3's metabolic roles, including energy metabolism, redox balance, and cellular signaling linked to α-KG-dependent processes. Mutations in IDH3G, though less studied than those in IDH1/2. have been implicated in metabolic disorders and cancers, where altered enzyme activity may disrupt mitochondrial function or promote oncogenesis via aberrant metabolite accumulation (e.g., 2-hydroxyglutarate).

Research on recombinant IDH3G also supports drug discovery, particularly for diseases linked to mitochondrial dysfunction. By analyzing its structure-function relationships or screening inhibitors, scientists aim to develop targeted therapies. Furthermore, recombinant IDH3G serves as a tool to investigate inherited deficiencies in the TCA cycle, providing insights into rare genetic disorders. Overall, IDH3G recombinant protein bridges molecular biology and clinical research, enhancing understanding of metabolic regulation and disease mechanisms.

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