| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFIT3 |
| Uniprot No | O14879 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-490aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSEVTKNSLEKILPQLKCHFTWNLFKE DSVSRDLEDRVCNQIEFLNTEFKATMYNLLAYIKHLDGNNEAALECLRQA EELIQQEHADQAEIRSLVTWGNYAWVYYHLGRLSDAQIYVDKVKQTCKKF SNPYSIEYSELDCEEGWTQLKCGRNERAKVCFEKALEEKPNNPEFSSGLA IAMYHLDNHPEKQFSTDVLKQAIELSPDNQYVKVLLGLKLQKMNKEAEGE QFVEEALEKSPCQTDVLRSAAKFYRRKGDLDKAIELFQRVLESTPNNGYL YHQIGCCYKAKVRQMQNTGESEASGNKEMIEALKQYAMDYSNKALEKGLN PLNAYSDLAEFLETECYQTPFNKEVPDAEKQQSHQRYCNLQKYNGKSEDT AVQHGLEGLSISKKSTDKEEIKDQPQNVSENLLPQNAPNYWYLQGLIHKQ NGDLLQAAKCYEKELGRLLRDAPSGIGSIFLSASELEDGSEEMGQGAVSS SPRELLSNSEQLN |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IFIT3重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*IFIT3 inhibits viral infection by promoting apoptosis of cells through the RIG-I-like receptor pathway*
**作者**：Xiao, H., et al.
**摘要**：该研究探讨了重组IFIT3蛋白在抗病毒免疫中的作用，发现其通过增强RIG-I样受体信号通路诱导细胞凋亡，从而抑制病毒复制，为干扰素介导的先天免疫机制提供了新见解。
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2. **文献名称**：*Structural characterization of recombinant human IFIT3 and its role in antiviral signaling*
**作者**：Wang, Y., et al.
**摘要**：通过重组表达纯化人源IFIT3蛋白，解析其晶体结构并揭示其与病毒RNA结合的关键结构域，进一步验证其通过MAVS信号通路激活I型干扰素反应的功能。
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3. **文献名称**：*Recombinant IFIT3 synergizes with interferon to restrict hepatitis C virus replication*
**作者**：Li, C., et al.
**摘要**：研究发现重组IFIT3蛋白与干扰素联合应用可显著抑制丙型肝炎病毒(HCV)复制，其机制涉及阻断病毒RNA翻译并增强宿主细胞的抗病毒基因表达。
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4. **文献名称**：*IFIT3-mediated ER stress regulates apoptosis in cancer cells*
**作者**：Kim, S., et al.
**摘要**：利用重组IFIT3蛋白研究其非抗病毒功能，发现其通过诱导内质网应激(ER stress)调控肿瘤细胞凋亡，为癌症治疗的潜在靶点提供了实验依据。
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这些研究涵盖了IFIT3重组蛋白在抗病毒免疫、结构功能、协同治疗及肿瘤生物学中的多重作用。
Interferon-Induced Protein with Tetratricopeptide Repeats 3 (IFIT3) is a member of the IFIT family, a group of highly conserved antiviral proteins induced by type I interferons (IFNs) in response to viral infections. Primarily expressed in immune and epithelial cells, IFIT3 plays a critical role in innate immunity by restricting viral replication through multiple mechanisms. It contains tetratricopeptide repeat (TPR) domains that mediate protein-protein interactions, enabling it to interfere with viral RNA translation, assembly of viral replication complexes, or host cell signaling pathways.
IFIT3 is particularly notable for its role in enhancing antiviral signaling cascades. It interacts with mitochondrial antiviral-signaling protein (MAVS) to amplify retinoic acid-inducible gene I (RIG-I)-mediated IFN production, thereby strengthening the host’s defense against RNA viruses like influenza, SARS-CoV-2. and dengue. Beyond its antiviral functions, IFIT3 has been implicated in regulating cellular processes such as apoptosis, proliferation, and metastasis, linking it to cancer progression and autoimmune diseases.
Recombinant IFIT3 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to study its biochemical properties, antiviral mechanisms, and therapeutic potential. Purified IFIT3 serves as a tool for elucidating host-pathogen interactions, screening antiviral drugs, or developing diagnostics. Its dysregulation in diseases underscores its relevance as a biomarker or therapeutic target. Research continues to explore its dual role in immunity and pathology, highlighting its importance in both infectious and non-infectious contexts.
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