| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | mns1B |
| Uniprot No | Q12563 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-513aa |
| 氨基酸序列 | YPHFGSSQPVLHSSSDTTQSRADAIKAAFSHAWDGYLQYAFPHDELHPVSNGYGDSRNGWGASAVDALSTAVIMRNATIVNQILDHVGKIDYSKTNTTVSLFETTIRYLGGMLSGYDLLKGPVSDLVQNSSKIDVLLTQSKNLADVLKFAFDTPSGVPYNNLNITSGGNDGAKTNGLAVTGTLALEWTRLSDLTGDTTYADLSQKAESYLLNPQPKSAEPFPGLVGSNINISNGQFTDAQVSWNGGDDSYYEYLIKMYVYDPKRFGLYKDRWVAAAQSTMQHLASHPSSRPDLTFLASYNNGTLGLSSQHLTCFDGGSFLLGGTVLNRTDFINFGLDLVSGCHDTYNSTLTGIGPESFSWDTSDIPSSQQSLYEKAGFYITSGAYILRPEVIESFYYAWRVTGQETYRDWIWSAFSAVNDYCRTSSGFSGLTDVNAANGGSRYDNQESFLFAEVMKYSYMAFAEDAAWQVQPGSGNQFVFNTEAHPVRVSST |
| 预测分子量 | 61.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MNS1B重组蛋白相关的文献示例(注：部分内容为模拟文献结构，具体引用请以实际文献为准)：
1. **文献名称**: "Functional characterization of recombinant MNS1B protein in ciliary assembly"
**作者**: Zhang L, et al.
**摘要**: 研究通过大肠杆菌系统表达重组MNS1B蛋白，发现其与纤毛微管结构蛋白相互作用，体外实验证明MNS1B通过调节TCTEX1D2复合物稳定性影响纤毛运动功能。
2. **文献名称**: "MNS1B knockout and rescue model reveals its role in sperm flagella formation"
**作者**: Tanaka K, et al.
**摘要**: 利用CRISPR构建MNS1B基因敲除小鼠模型，发现精子鞭毛结构异常导致不育。通过重组MNS1B蛋白回补实验证实其通过结合SPAG6/SPAG16L复合物调控鞭毛轴丝组装。
3. **文献名称**: "Structural analysis of MNS1B dimerization domain by X-ray crystallography"
**作者**: Wang Y, et al.
**摘要**: 解析了重组人源MNS1B蛋白的N端结构域晶体结构(分辨率2.1Å)，揭示其通过α-螺旋疏水核心形成同源二聚体，为开发靶向MNS1B的男性避孕药物提供结构基础。
4. **文献名称**: "MNS1B recombinant protein alleviates primary ciliary dyskinesia in zebrafish"
**作者**: Chen R, et al.
**摘要**: 在MNS1B缺陷斑马鱼模型中，外源性注射重组MNS1B蛋白可部分恢复纤毛摆动频率，并通过免疫荧光证实其定位于纤毛基体，提示其治疗纤毛运动障碍疾病的潜力。
注：以上文献信息为模拟生成，实际研究中建议通过PubMed或Web of Science以“MNS1B recombinant protein”为关键词检索最新文献。部分研究可能以“MNS1”(非B亚型)为主，需注意区分亚型特异性。
The MNS1B recombinant protein is derived from the MNS1 (Meiosis-Specific Nuclear Structural protein 1) gene, which plays a critical role in ciliogenesis, sperm flagella formation, and cellular motility. Initially identified for its meiosis-specific expression, MNS1 is evolutionarily conserved and shares homology with proteins involved in microtubule dynamics and ciliary function. The MNS1B isoform, alternatively spliced, encodes a protein containing coiled-coil domains and transmembrane regions, suggesting its involvement in structural organization and membrane-associated processes.
Research highlights MNS1B's essential role in male fertility, as knockout models exhibit impaired sperm motility due to defective flagella assembly. It interacts with intraflagellar transport (IFT) complexes, facilitating cargo delivery during cilia/flagella biogenesis. Beyond reproduction, MNS1B contributes to primary cilia function in somatic cells, influencing Hedgehog signaling and mechanosensory pathways implicated in development and tissue homeostasis.
Recombinant MNS1B proteins are typically produced in heterologous systems (e.g., E. coli, mammalian cells) for functional studies. These tools enable investigations into protein-protein interactions, post-translational modifications, and structural characterization. Current applications include elucidating molecular mechanisms of ciliopathies, infertility disorders, and potential roles in cancer metastasis where ciliary dysfunction or cell motility pathways are dysregulated. Its recombinant form also serves as an antigen for antibody development, aiding diagnostic and therapeutic research targeting cilia-related pathologies.
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