| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ORC1 |
| Uniprot No | Q13415 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-861aa |
| 氨基酸序列 | MAHYPTRLKTRKTYSWVGRPLLDRKLHYQTYREMCVKTEGCSTEIHIQIGQFVLIEGDDDENPYVAKLLELFEDDSDPPPKKRARVQWFVRFCEVPACKRHLLGRKPGAQEIFWYDYPACDSNINAETIIGLVRVIPLAPKDVVPTNLKNEKTLFVKLSWNEKKFRPLSSELFAELNKPQESAAKCQKPVRAKSKSAESPSWTPAEHVAKRIESRHSASKSRQTPTHPLTPRARKRLELGNLGNPQMSQQTSCASLDSPGRIKRKVAFSEITSPSKRSQPDKLQTLSPALKAPEKTRETGLSYTEDDKKASPEHRIILRTRIAASKTIDIREERTLTPISGGQRSSVVPSVILKPENIKKRDAKEAKAQNEATSTPHRIRRKSSVLTMNRIRQQLRFLGNSKSDQEEKEILPAAEISDSSSDEEEASTPPLPRRAPRTVSRNLRSSLKSSLHTLTKVPKKSLKPRTPRCAAPQIRSRSLAAQEPASVLEEARLRLHVSAVPESLPCREQEFQDIYNFVESKLLDHTGGCMYISGVPGTGKTATVHEVIRCLQQAAQANDVPPFQYIEVNGMKLTEPHQVYVQILQKLTGQKATANHAAELLAKQFCTRGSPQETTVLLVDELDLLWTHKQDIMYNLFDWPTHKEARLVVLAIANTMDLPERIMMNRVSSRLGLTRMCFQPYTYSQLQQILRSRLKHLKAFEDDAIQLVARKVAALSGDARRCLDICRRATEICEFSQQKPDSPGLVTIAHSMEAVDEMFSSSYITAIKNSSVLEQSFLRAILAEFRRSGLEEATFQQIYSQHVALCRMEGLPYPTMSETMAVCSHLGSCRLLLVEPSRNDLLLRVRLNVSQDDVLYALKDE |
| 预测分子量 | 97.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ORC1重组蛋白研究的参考文献摘要：
---
1. **文献名称**：*Reconstitution of recombinant human ORC1 reveals its role in DNA replication origin licensing*
**作者**：Siddiqui et al.
**摘要**：该研究通过重组表达纯化人源ORC1蛋白，结合体外实验证明ORC1在DNA复制起始位点许可(origin licensing)中的关键作用，揭示了其与染色质结合及与其他复制起始复合物组分的相互作用机制。
---
2. **文献名称**：*Structural analysis of the ORC1 protein in the origin recognition complex*
**作者**：Bleichert et al.
**摘要**：通过重组ORC1蛋白的结晶和结构解析，揭示了ORC1在复制起始复合体(ORC)中的构象变化及其与ATP结合的结构基础，为理解DNA复制起始的分子机制提供了重要依据。
---
3. **文献名称**：*Functional characterization of ORC1 mutations in Meier-Gorlin syndrome*
**作者**：Guernsey et al.
**摘要**：利用重组ORC1蛋白进行功能研究，发现导致Meier-Gorlin综合征的ORC1突变会破坏其与DNA及其他复制起始因子的结合能力，阐明了该疾病的分子病理机制。
---
(注：以上文献信息为示例，实际文献需根据具体研究内容检索。)
**Background of ORC1 Recombinant Protein**
The Origin Recognition Complex subunit 1 (ORC1) is a critical component of the Origin Recognition Complex (ORC), a highly conserved eukaryotic protein complex essential for initiating DNA replication. ORC1 binds to replication origins during the G1 phase of the cell cycle, serving as a scaffold for assembling pre-replication complexes (pre-RCs) by recruiting CDC6. CDT1. and the minichromosome maintenance (MCM) helicase. This process ensures precise duplication of genomic material during S phase, maintaining genome stability.
ORC1’s role extends beyond replication initiation. It participates in chromatin remodeling, transcriptional silencing, and cell cycle regulation. Structurally, ORC1 contains AAA+ ATPase and winged-helix domains, enabling ATP-dependent interactions with DNA and other ORC subunits (ORC2-6). Dysregulation of ORC1 is linked to developmental disorders like Meier-Gorlin syndrome (growth defects) and cancers, often due to mutations disrupting replication licensing.
Recombinant ORC1 protein, produced via heterologous expression systems (e.g., *E. coli* or insect cells), retains biochemical activity and is widely used to study replication mechanisms *in vitro*. Purified ORC1 enables analyses of DNA binding, ATP hydrolysis, and interactions with replication factors. It also aids structural studies (e.g., cryo-EM) to resolve ORC architecture and dynamics. Additionally, recombinant ORC1 supports drug discovery targeting replication abnormalities in cancer.
Research using ORC1 recombinant protein has clarified its role in replication timing, epigenetic inheritance, and replication stress responses. These insights advance understanding of cell proliferation, differentiation, and disease pathogenesis, positioning ORC1 as a focal point in both basic and applied biomedical research.
×
