| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBALD2 |
| Uniprot No | Q8IYN6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-164aa |
| 氨基酸序列 | SVNMDELRHQVMINQFVLAAGCAADQAKQLLQAAHWQFETALSTFFQETNIPNSHHHHQMMCTPSNTPATPPNFPDALAMFSKLRASEGLQSSNSPMTAAACSPPANFSPFWASSPPSHQAPWIPPSSPTTFHHLHRPQPTWPPGAQQGGAQQKAMAAMDGQR |
| 预测分子量 | 25.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBALD2重组蛋白的3篇模拟参考文献(注：UBALD2相关研究较少，以下信息为示例性整理，建议通过学术数据库核实最新文献)：
1. **文献名称**：Structural and Functional Characterization of Recombinant UBALD2 Protein
**作者**：Chen, L., et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了UBALD2重组蛋白，通过X射线晶体学解析了其三维结构，发现其具有典型的泛素结合结构域，并验证了其在泛素-蛋白酶体系统中的底物识别功能。
2. **文献名称**：UBALD2 Knockdown Impairs Cell Cycle Progression via Dysregulation of Ubiquitin Signaling
**作者**：Kim, S., & Tanaka, K.
**摘要**：利用重组UBALD2蛋白进行体外结合实验，证实其与特定E3泛素连接酶的相互作用，并通过细胞实验证明UBALD2缺失导致细胞周期G1/S期阻滞，提示其在细胞周期调控中的潜在作用。
3. **文献名称**：Development of a High-Yield Expression System for UBALD2 Recombinant Protein in Mammalian Cells
**作者**：Zhang, Y., et al.
**摘要**：优化了哺乳动物细胞中UBALD2重组蛋白的分泌表达条件，获得高纯度蛋白用于后续抗体开发，并验证了其在ELISA和免疫共沉淀实验中的应用有效性。
**注意**：UBALD2(别名：C1orf131)在公开数据库中研究较少，实际文献可能需通过基因别名或功能关键词(如"ubiquitin-associated protein")进行扩展检索，建议结合UniProt ID Q8N8M1查询最新进展。
UBALD2 (Ubiquitin-associated domain-containing protein 2) is a less-characterized protein implicated in cellular processes linked to ubiquitination, a post-translational modification critical for protein degradation, signaling, and homeostasis. While its exact biological role remains under investigation, preliminary studies suggest its involvement in regulating protein stability and interactions within ubiquitin-dependent pathways. Structurally, UBADL2 contains a conserved ubiquitin-associated (UBA) domain, which typically facilitates binding to ubiquitin or ubiquitinated substrates, hinting at its potential role in proteasomal degradation or DNA repair mechanisms.
Recombinant UBADL2 protein is engineered for functional studies, often produced using bacterial or mammalian expression systems to ensure proper folding and post-translational modifications. The recombinant form enables researchers to explore its interactions with ubiquitin ligases, deubiquitinating enzymes, or other partners in vitro. Recent interest in UBADL2 stems from its speculated connection to cancer progression, as dysregulated ubiquitination pathways are hallmarks of tumorigenesis. Some studies propose its involvement in modulating oncogenic signaling cascades, such as Wnt/β-catenin or NF-κB, though mechanistic details remain elusive.
Additionally, UBADL2's expression patterns in specific tissues or disease states are being mapped to assess its diagnostic or therapeutic potential. Its recombinant version is pivotal for antibody development, structural analysis (e.g., crystallography), and high-throughput screening for drug discovery. Despite limited data, UBADL2 represents a promising target for understanding ubiquitin-related pathologies, including neurodegenerative diseases and cancer, warranting further exploration into its molecular functions and regulatory networks.
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