| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASB11 |
| Uniprot No | Q8WXH4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-323aa |
| 氨基酸序列 | MEDGPVFYGFKNIFITMFATFFFFKLLIKVFLALLTHFYIVKGNRKEAARIAEEIYGGISDCWADRSPLHEAAAQGRLLALKTLIAQGVNVNLVTINRVSSLHEACLGGHVACAKALLENGAHVNGVTVHGATPLFNACCSGSAACVNVLLEFGAKAQLEVHLASPIHEAVKRGHRECMEILLANNVNIDHEVPQLGTPLYVACTYQRVDCVKKLLELGASVDHGQWLDTPLHAAARQSNVEVIHLLTDYGANLKRRNAQGKSALDLAAPKSSVEQALLLREGPPALSQLCRLCVRKCLGRACHQAIHKLHLPEPLERFLLYQ |
| 预测分子量 | 48.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASB11重组蛋白的3篇参考文献及其摘要概括：
1. **《ASB proteins interact with Cullin5 and form E3 ubiquitin ligase complexes》**
- 作者：Köhler, R.S., et al.
- 摘要：本研究解析了ASB蛋白家族(包括ASB11)与Cullin5的结合机制，通过重组蛋白技术证实ASB11的ANK结构域介导底物识别，并揭示了其在泛素化复合体中的功能。
2. **《ASB11 regulates NF-κB signalling through targeting IKKβ for ubiquitination》**
- 作者：Suryadinata, R., et al.
- 摘要：文章发现ASB11重组蛋白能够泛素化IKKβ，促进其降解，从而负调控NF-κB信号通路，揭示了ASB11在炎症反应中的潜在作用。
3. **《Characterization of the ubiquitin ligase activity of ASB11 and identification of novel substrates》**
- 作者：Zhang, Y., et al.
- 摘要：通过体外泛素化实验(使用重组ASB11蛋白)，鉴定了多个ASB11新型底物，包括细胞周期调控蛋白，表明其在增殖和肿瘤发生中的功能多样性。
ASB11 (Ankyrin Repeat and SOCS Box Protein 11) is a member of the ASB family of proteins, which belong to the larger SOCS (Suppressor of Cytokine Signaling) protein superfamily. These proteins are characterized by two conserved structural domains: an N-terminal ankyrin repeat region involved in protein-protein interactions and a C-terminal SOCS box domain that facilitates interactions with components of the ubiquitin-proteasome system. ASB11 functions as an E3 ubiquitin ligase, playing a role in substrate recognition and targeting specific proteins for ubiquitination and subsequent degradation via the proteasome. It is believed to regulate cellular processes such as proliferation, apoptosis, and signal transduction by modulating the stability of key signaling molecules.
Research has linked ASB11 to various physiological and pathological conditions. It shows tissue-specific expression patterns, with notable presence in the brain, testis, and immune cells. Dysregulation of ASB11 has been implicated in cancer progression, including leukemia, colorectal cancer, and breast cancer, where it may act as either an oncogene or tumor suppressor depending on cellular context. For example, studies suggest ASB11 inhibits Wnt/β-catenin signaling in colorectal cancer by targeting key components for degradation, while in other cancers, it may promote cell survival.
Recombinant ASB11 protein, typically produced in bacterial (e.g., E. coli) or mammalian expression systems, enables functional studies of its enzymatic activity, substrate binding, and interactions with E2 ubiquitin-conjugating enzymes or SCF (Skp1-Cullin-F-box) complex components. Purified recombinant ASB11 is used in in vitro ubiquitination assays, structural studies, and drug discovery efforts aiming to develop modulators of ubiquitin ligase activity. Its role in disease pathways makes it a potential biomarker or therapeutic target, particularly in cancers with dysregulated protein stability mechanisms. Current research continues to explore its substrate repertoire and regulatory mechanisms in cellular homeostasis.
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