| 纯度 | >97%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL10RA |
| Uniprot No | Q13651 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-235aa |
| 氨基酸序列 | HGTELPSPP SVWFEAEFFH HILHWTPIPN QSESTCYEVA LLRYGIESWN SISNCSQTLS YDLTAVTLDL YHSNGYRARV RAVDGSRHSN WTVTNTRFSV DEVTLTVGSV NLEIHNGFIL GKIQLPRPKM APANDTYESI FSHFREYEIA IRKVPGNFTF THKKVKHENF SLLTSGEVGE FCVQVKPSVA SRSNKGMWSK EECISLTRQY FTVTN |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL10RA重组蛋白的3篇示例参考文献(内容为虚构示例，供参考)：
1. **文献名称**: "Structural and functional characterization of recombinant human IL10RA protein"
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人IL10RA蛋白，通过X射线晶体学解析了其三维结构，证实其与IL10的结合域关键位点，为炎症性疾病治疗提供了结构基础。
2. **文献名称**: "IL10RA signaling modulation in autoimmune disorders using engineered recombinant proteins"
**作者**: Zhang L, et al.
**摘要**: 通过基因工程改造IL10RA重组蛋白，验证其在体外抑制巨噬细胞过度炎症反应的能力，并在小鼠模型中缓解了类风湿性关节炎症状，表明其潜在治疗价值。
3. **文献名称**: "High-yield production of bioactive IL10RA-Fc fusion protein in mammalian cells"
**作者**: Kim S, et al.
**摘要**: 开发了一种CHO细胞表达系统，规模化生产具有Fc标签的IL10RA重组融合蛋白，证实其增强的稳定性和体外IL10信号通路激活能力，适用于生物制药开发。
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建议通过PubMed或Google Scholar搜索关键词 **"IL10RA recombinant protein"** 或 **"IL10 receptor alpha expression"** 获取真实文献。
Interleukin-10 receptor alpha (IL10RA) is a critical component of the interleukin-10 (IL-10) signaling pathway, which plays a pivotal role in modulating immune responses and maintaining inflammatory homeostasis. As a type II transmembrane glycoprotein, IL10RA belongs to the class II cytokine receptor family and forms a heterodimeric complex with IL10RB to bind IL-10 with high affinity. This interaction activates downstream signaling cascades, primarily through the JAK-STAT pathway, to suppress pro-inflammatory cytokine production and enhance anti-inflammatory responses. Dysregulation of IL10RA is implicated in chronic inflammatory diseases, autoimmune disorders, and certain cancers.
Recombinant IL10RA protein is engineered using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications and functional activity. It typically includes the extracellular domain of IL10RA, which mediates ligand binding and receptor assembly. Purification techniques like affinity chromatography yield high-purity proteins suitable for structural studies, ligand-receptor interaction assays, and therapeutic development. Researchers utilize recombinant IL10RA to investigate mechanisms of IL-10-mediated immune tolerance, screen for receptor agonists/antagonists, and explore its therapeutic potential in conditions like inflammatory bowel disease (IBD) or psoriasis. Mutations in IL10RA are also linked to early-onset IBD in humans, making this recombinant protein valuable for studying genetic defects and personalized treatment strategies. Its applications extend to in vitro models for testing immunomodulatory drugs and biomarker discovery.
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