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| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL-4 |
| Uniprot No | P05112 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-153aa |
| 氨基酸序列 | HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS |
| 预测分子量 | 46.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于IL-4重组蛋白的参考文献示例(注：文献信息为模拟生成，供参考)：
1. **"High-level expression and purification of recombinant human IL-4 in Escherichia coli"**
- 作者：A. Miyajima et al.
- 摘要：报道了利用大肠杆菌表达系统高效表达人源IL-4重组蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并验证其生物活性。
2. **"Structural and functional analysis of IL-4 mutants reveals key residues for receptor binding"**
- 作者：W.E. Paul et al.
- 摘要：通过定点突变技术研究IL-4重组蛋白的结构与功能关系，鉴定出与IL-4受体α链结合的关键氨基酸位点，为靶向药物设计提供依据。
3. **"IL-4 recombinant protein enhances antitumor immunity by polarizing macrophages to M1 phenotype"**
- 作者：P. Conti et al.
- 摘要：实验证明IL-4重组蛋白通过调控巨噬细胞极化状态(M1/M2平衡)增强抗肿瘤免疫反应，为癌症免疫治疗提供新策略。
4. **"Delivery of IL-4 plasmid DNA via biodegradable nanoparticles ameliorates allergic asthma in mice"**
- 作者：Y. Li et al.
- 摘要：开发了一种基于纳米颗粒的IL-4重组蛋白/基因递送系统，在哮喘小鼠模型中显著减轻气道炎症和Th2免疫应答。
(注：以上文献标题及摘要内容基于领域内典型研究方向模拟，实际引用需核对真实文献数据库)
Interleukin-4 (IL-4) is a pleiotropic cytokine belonging to the interleukin family, primarily produced by activated T-helper 2 (Th2) cells, mast cells, and basophils. It plays a pivotal role in regulating immune responses, particularly in promoting humoral immunity, B-cell proliferation, and antibody class-switching to IgE. IL-4 also drives the differentiation of naïve T cells into Th2 cells, contributing to anti-inflammatory and tissue repair pathways while counterbalancing Th1-mediated pro-inflammatory responses. Dysregulation of IL-4 signaling is implicated in allergic diseases, asthma, autoimmune disorders, and certain cancers.
Recombinant IL-4 proteins are engineered using genetic modification techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems (e.g., CHO cells) to ensure proper folding and bioactivity. These proteins retain the native structure and functional epitopes of endogenous IL-4. enabling researchers to study its mechanisms or develop therapeutic strategies. Recombinant IL-4 is widely utilized in *in vitro* and *in vivo* models to investigate immune cell differentiation, cytokine networks, and disease pathogenesis. Its clinical relevance extends to experimental therapies targeting IL-4 receptors, such as monoclonal antibodies or fusion proteins designed to modulate allergic or oncogenic pathways.
Structurally, IL-4 is a four-helix bundle protein binding to type I/II receptor complexes, initiating JAK-STAT signaling cascades (notably STAT6 activation). Recombinant variants may include tags (e.g., His, Fc) for purification or functional studies. Quality control involves rigorous testing for endotoxin levels, bioactivity in cell proliferation assays, and structural validation via SDS-PAGE or mass spectrometry. As research advances, IL-4 recombinant proteins remain critical tools for dissecting immune regulation and developing precision immunotherapies.
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