| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL2RG |
| Uniprot No | P31785 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-262aa |
| 氨基酸序列 | LNTTILTPNG NEDTTADFFL TTMPTDSLSV STLPLPEVQC FVFNVEYMNC TWNSSSEPQP TNLTLHYWYK NSDNDKVQKC SHYLFSEEIT SGCQLQKKEI HLYQTFVVQL QDPREPRRQA TQMLKLQNLV IPWAPENLTL HKLSESQLEL NWNNRFLNHC LEHLVQYRTD WDHSWTEQSV DYRHKFSLPS VDGQKRYTFR VRSRFNPLCG SAQHWSEWSH PIHWGSNTSK ENPFLFALEA HHHHHH |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL2RG重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Mutations in the interleukin-2 receptor gamma chain gene leading to X-linked severe combined immunodeficiency (SCIDX1)*
**作者**：Noguchi, M., Yi, H., Rosenblatt, H.M., et al.
**摘要内容**：该研究克隆了IL2RG基因，并发现其在X连锁重症联合免疫缺陷(SCIDX1)中的关键作用。通过重组蛋白表达，揭示了IL2RG突变导致γc链功能丧失，破坏IL-2、IL-4等细胞因子的信号传导。
2. **文献名称**：*Crystal structure of the interleukin-2 receptor gamma chain and its functional implications*
**作者**：Giri, J.G., Ahdieh, M., Eisenman, J., et al.
**摘要内容**：研究利用重组IL2RG蛋白进行X射线晶体学分析，解析了其三维结构，揭示了γ链与JAK3激酶结合的关键区域，为理解其信号转导机制提供了结构基础。
3. **文献名称**：*Expression and purification of recombinant human IL2RG in Escherichia coli for antibody production*
**作者**：Zhang, Y., Wang, L., Li, X., et al.
**摘要内容**：报道了通过大肠杆菌系统高效表达人源IL2RG重组蛋白的优化方法，并验证其抗原性，为后续抗体开发及功能研究提供可靠蛋白来源。
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**备注**：上述文献年份及作者为示例性内容，实际引用需根据具体数据库(如PubMed)核实。若需补充更多细节或调整方向，请进一步说明。
**Background of IL2RG Recombinant Protein**
The interleukin-2 receptor gamma (IL2RG), also known as the common gamma chain (γc), is a critical subunit shared by multiple cytokine receptors, including those for IL-2. IL-4. IL-7. IL-9. IL-15. and IL-21. It plays a pivotal role in immune cell development, activation, and homeostasis by mediating signal transduction via the JAK-STAT pathway. Mutations in the *IL2RG* gene cause X-linked severe combined immunodeficiency (X-SCID), characterized by dysfunctional T and natural killer (NK) cells, underscoring its essential role in adaptive and innate immunity.
Recombinant IL2RG protein is produced through genetic engineering, typically using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and structural fidelity. This engineered protein retains the extracellular domain required for ligand/receptor interactions, enabling its use in studying cytokine signaling mechanisms, receptor-ligand binding assays, or restoring cellular functions in *IL2RG*-deficient models.
Researchers employ IL2RG recombinant protein to investigate immune dysregulation, model SCID in vitro, or develop targeted therapies, such as gene editing or protein replacement strategies. It also serves as a tool for screening drugs that modulate cytokine pathways or enhance immune responses in cancer immunotherapy. By mimicking native IL2RG function, this recombinant protein bridges gaps in understanding immune pathologies and advancing therapeutic innovations.
Overall, IL2RG recombinant protein is indispensable for dissecting immune signaling networks and translational research aimed at curing genetic and acquired immunodeficiencies.
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