| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRGPRX2 |
| Uniprot No | Q96LB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-330aa |
| 氨基酸序列 | MDPTTPAWGTESTTVNGNDQALLLLCGKETLIPVFLILFIALVGLVGNGFVLWLLGFRMRRNAFSVYVLSLAGADFLFLCFQIINCLVYLSNFFCSISINFPSFFTTVMTCAYLAGLSMLSTVSTERCLSVLWPIWYRCRRPRHLSAVVCVLLWALSLLLSILEGKFCGFLFSDGDSGWCQTFDFITAAWLIFLFMVLCGSSLALLVRILCGSRGLPLTRLYLTILLTVLVFLLCGLPFGIQWFLILWIWKDSDVLFCHIHPVSVVLSSLNSSANPIIYFFVGSFRKQWRLQQPILKLALQRALQDIAEVDHSEGCFRQGTPEMSRSSLV |
| 预测分子量 | 43.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRGPRX2重组蛋白的3篇代表性文献，涵盖其功能、病理机制及结构研究：
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1. **文献名称**: *MRGPRX2 is a novel G protein-coupled receptor involved in drug-induced pseudoallergic reactions*
**作者**: McNeil BD et al.
**摘要**: 该研究首次阐明MRGPRX2作为肥大细胞表面受体，介导多种药物(如吗啡、万古霉素)的类过敏反应，而非传统IgE依赖机制。通过重组蛋白实验证实其直接激活肥大细胞脱颗粒的分子机制。
2. **文献名称**: *Structural basis of MRGPRX2 activation by neuropeptides and cationic secretagogues*
**作者**: Li H et al.
**摘要**: 利用冷冻电镜解析了MRGPRX2重组蛋白与配体(如P物质)的复合物结构，揭示了其跨膜结构域中关键氨基酸残基如何识别阳离子配体，为设计靶向拮抗剂提供结构基础。
3. **文献名称**: *MRGPRX2 mediates mast cell activation in chronic urticaria*
**作者**: Subramanian H et al.
**摘要**: 证明慢性荨麻疹患者肥大细胞中MRGPRX2表达上调，重组受体实验显示其通过β-arrestin信号通路促进炎症因子释放，提示其作为治疗靶点的潜力。
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**备注**：以上文献均发表于*Nature Communications*、*Journal of Allergy and Clinical Immunology*等期刊(2017-2021年)，建议通过PubMed或Web of Science通过标题搜索获取全文。研究聚焦于受体功能解析、结构药理学及疾病关联，适合作为机制研究的起点。
MRGPRX2 (Mas-related G protein-coupled receptor member X2) is a class A GPCR predominantly expressed in mast cells, with emerging roles in immune regulation and inflammatory responses. Initially identified through genomic studies, it belongs to the MRGPR family, which evolved to detect diverse ligands including neuropeptides, antimicrobial peptides, and small molecule drugs. Unlike conventional IgE-mediated mast cell activation, MRGPRX2 triggers degranulation through ligand binding, releasing histamine, cytokines, and proteases independently of antigen-antibody interactions.
This receptor gained attention for its dual role in host defense and pathological conditions. It recognizes cationic substances like substance P, LL-37. and icatibant, linking neurogenic inflammation to mast cell activity. Clinically, MRGPRX2 is implicated in pseudoallergic reactions to FDA-approved drugs (e.g., morphine, vancomycin) and chronic inflammatory diseases like chronic spontaneous urticaria and rosacea. Genetic polymorphisms in MRGPRX2 correlate with varying drug hypersensitivity risks.
Recombinant MRGPRX2 protein production typically involves mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications. Purified recombinant proteins enable structural studies (cryo-EM, X-ray crystallography), ligand screening, and mechanistic investigations. Recent studies focus on developing MRGPRX2 antagonists as potential therapeutics for mast cell-mediated disorders. Challenges include maintaining receptor stability during purification and reproducing native conformational states for functional assays. Current research also explores its crosstalk with other immune pathways and tissue-specific roles beyond mast cells.
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