| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSN1S1 |
| Uniprot No | P47710 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-185aa |
| 氨基酸序列 | RPKLPLRYPERLQNPSESSEPIPLESREEYMNGMNRQRNILREKQTDEIKDTRNESTQNCVVAEPEKMESSISSSSEEMSLSKCAEQFCRLNEYNQLQLQAAHAQEQIRRMNENSHVQVPFQQLNQLAAYPYAVWYYPQIMQYVPFPPFSDISNPTAHENYEKNNVMLQW |
| 预测分子量 | 26.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSN1S1重组蛋白的3篇文献示例(内容基于公开研究归纳，非真实文献)：
1. **文献名称**：*Heterologous Expression and Purification of Recombinant Bovine αs1-Casein (CSN1S1) in E. coli*
**作者**：Smith A, et al.
**摘要**：本研究利用大肠杆菌表达系统成功表达了牛源CSN1S1重组蛋白，通过优化密码子和诱导条件提高蛋白溶解度，并采用亲和层析技术纯化，验证了其结构与天然酪蛋白的相似性。
2. **文献名称**：*Functional Characterization of Recombinant CSN1S1 in Milk Emulsification*
**作者**：Zhang L, et al.
**摘要**：通过酵母表达系统制备重组CSN1S1.分析其乳化稳定性及钙结合能力，证明重组蛋白在乳脂微粒形成中具有与天然蛋白相当的生物活性，为乳制品加工提供新思路。
3. **文献名称**：*CSN1S1 Recombinant Protein as a Potential Carrier for Nutraceutical Delivery*
**作者**：Gupta R, et al.
**摘要**：探讨了重组CSN1S1蛋白的载药能力，发现其自组装特性可包裹疏水性营养素，在模拟消化环境中缓释，表明其在功能食品或药物递送中的应用潜力。
(注：以上为模拟文献，实际引用请通过学术数据库检索真实发表的研究。)
CSN1S1. also known as αs1-casein, is a major milk protein component in mammals, playing a critical role in nutrient delivery and structural organization of casein micelles in milk. As one of the four primary caseins (αs1. αs2. β, κ), it contributes to milk's colloidal stability, calcium phosphate transport, and digestion properties. In humans and dairy species, CSN1S1 is encoded by the CSN1S1 gene and synthesized in mammary epithelial cells.
Recombinant CSN1S1 protein is produced using biotechnological platforms (e.g., bacterial, yeast, or mammalian expression systems) to replicate the native protein's structure and function. This engineered approach enables large-scale production without relying on animal sources, addressing ethical concerns and supply limitations. The recombinant protein retains key features like phosphorylation sites and hydrophobic domains essential for micelle formation, though post-translational modifications may require optimized expression systems.
Research applications include studying milk protein allergies (CSN1S1 is a common allergen in cow's milk), developing hypoallergenic infant formulas, and creating functional food additives. In biotechnology, recombinant CSN1S1 serves as a model for protein self-assembly mechanisms and drug delivery vehicle design due to its natural nanocarrier properties. Recent studies also explore its potential in biomaterials and nutraceuticals. Production challenges involve achieving proper folding and post-translational modifications to match native protein functionality, driving ongoing optimization of expression platforms.
×
