| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | snrnp35 |
| Uniprot No | Q4KMD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208aa |
| 氨基酸序列 | MEWSPVAKVYDPLKAGSIDGTDVEPHDAGVWRAMLARYKPNRGVCGDPDLTLFVARLNPQTTEEKLRDVFSKFGDIRRLRLVRDVVTGFSKRYAFIEYKEERSLKRAWRDANKLILDQYELLVDVEQERTLPGWRPRRLGGGQGGQKESGQLRFGGRDRPFRKPINLSTRRPAEPRGRETERERDRRDYRDRRHERTHTEDRTHRHTY |
| 预测分子量 | 28.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与SNRNP35重组蛋白相关的参考文献示例(注：SNRNP35研究较为小众，部分文献可能涉及其功能或重组技术，以下为模拟示例)：
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1. **文献名称**: "SNRPN35 is a component of the U12-type spliceosome essential for splicing fidelity"
**作者**: Li et al. (2018)
**摘要**: 研究揭示了SNRNP35作为U12型剪接体的核心组分，通过重组蛋白表达验证其与U12 snRNA的相互作用，并证明其缺失导致次要内含子剪接异常。
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2. **文献名称**: "Recombinant SNRNP35 facilitates in vitro assembly of the U11/U12 di-snRNP complex"
**作者**: Zhang & Jurica (2020)
**摘要**: 报道了重组SNRNP35蛋白在大肠杆菌中的高效表达与纯化，证实其与U11/U12 snRNP复合体的体外组装能力，为剪接体机制研究提供工具。
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3. **文献名称**: "Structural insights into SNRNP35's role in spliceosomal B complex formation"
**作者**: Kim et al. (2021)
**摘要**: 利用重组SNRNP35进行冷冻电镜分析，解析其与剪接体B复合体中其他蛋白(如DDX23)的结合界面，阐明其在剪接体动态组装中的构象变化。
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4. **文献名称**: "SNRNP35 mutations linked to neurodegenerative disorders impair recombinant protein stability"
**作者**: Wang et al. (2022)
**摘要**: 发现神经退行性疾病相关SNRNP35突变体在重组表达中易聚集，导致剪接功能缺陷，提示其病理机制可能与蛋白稳定性下降有关。
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**说明**：以上文献为模拟示例，实际研究中需通过PubMed或Web of Science以“SNRNP35”、“U11/U12 snRNP”或“minor spliceosome”为关键词检索最新文献。
SNRNP35 (Small Nuclear Ribonucleoprotein 35 kDa), also known as SNRPA1 or U1-A, is a critical component of the spliceosome, a dynamic molecular machinery responsible for pre-mRNA splicing in eukaryotic cells. It belongs to the U1 small nuclear ribonucleoprotein (snRNP) complex, which recognizes the 5' splice site of introns during the early stages of spliceosome assembly. The protein contains an RNA recognition motif (RRM) that binds specifically to the stem-loop II region of U1 snRNA, stabilizing the interaction between U1 snRNP and pre-mRNA. This interaction is essential for accurate splice site selection and subsequent excision of introns.
Recombinant SNRNP35 protein is engineered through heterologous expression systems (e.g., E. coli or mammalian cells) to study its structural and functional properties. Its production typically involves cloning the SNRPA1 gene into expression vectors, followed by affinity chromatography purification using tags like His-tag or GST. The recombinant protein retains RNA-binding activity and can be used in in vitro splicing assays, protein-RNA interaction studies, and structural analyses.
Research on SNRNP35 has implications for understanding splicing dysregulation in diseases, including cancers and neurodegenerative disorders. Mutations or altered expression of spliceosome components, including SNRNP35. have been linked to aberrant mRNA processing and disease pathogenesis. Recombinant SNRNP35 also serves as a tool for developing small-molecule modulators targeting spliceosome activity, a growing area in therapeutic development. Current studies focus on resolving its 3D structure, post-translational modifications, and interactions with other splicing factors to elucidate mechanistic details of spliceosome dynamics.
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