| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rep78 |
| Uniprot No | Q9NXV2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234aa |
| 氨基酸序列 | MAENHCELLSPARGGIGAGLGGGLCRRCSAGLGALAQRPGSVSKWVRLNVGGTYFLTTRQTLCRDPKSFLYRLCQADPDLDSDKDETGAYLIDRDPTYFGPVLNYLRHGKLVINKDLAEEGVLEEAEFYNITSLIKLVKDKIRERDSKTSQVPVKHVYRVLQCQEEELTQMVSTMSDGWKFEQLVSIGSSYNYGNEDQAEFLCVVSKELHNTPYGTASEPSEKAKILQERGSRM |
| 预测分子量 | 26kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于AAV Rep78重组蛋白的模拟参考文献(内容基于领域研究热点概括，非真实文献)：
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1. **《AAV Rep78蛋白在病毒基因组复制中的功能研究》**
- 作者：Linden, R.M. 等
- 摘要：研究Rep78重组蛋白在腺相关病毒(AAV)生命周期中的作用，揭示其通过结合病毒ITR序列调控DNA复制和位点特异性整合的分子机制。
2. **《重组Rep78蛋白在基因治疗载体设计中的应用》**
- 作者：Muzyczka, N. 等
- 摘要：探讨利用重组Rep78蛋白增强AAV载体生产效率和靶向基因组整合的策略，为优化基因治疗载体提供理论支持。
3. **《Rep78蛋白的ATP酶活性与解旋酶功能的结构解析》**
- 作者：James, J.A. 等
- 摘要：通过晶体学分析Rep78重组蛋白的ATP结合域和解旋酶活性区域，阐明其在病毒DNA复制中的能量依赖型功能机制。
4. **《Rep78与宿主细胞蛋白相互作用的调控网络》**
- 作者：Kleinschmidt, J.A. 等
- 摘要：鉴定Rep78重组蛋白与宿主细胞因子(如PCNA、p53)的相互作用，揭示其影响病毒潜伏期和细胞周期调控的分子路径。
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注：以上文献为模拟概括，实际研究需参考真实发表的论文(如《Journal of Virology》《Gene Therapy》等期刊)。
**Background of Rep78 Recombinant Protein**
Rep78 is a multifunctional regulatory protein derived from the adeno-associated virus (AAV), a small, non-pathogenic member of the *Parvoviridae* family. AAV relies on helper viruses (e.g., adenovirus or herpesvirus) for efficient replication in host cells. Rep78. encoded by the AAV *rep* gene, plays a central role in viral genome replication, site-specific integration into the host genome, and regulation of viral gene expression. It exhibits helicase and ATPase activities, enabling it to bind specific DNA sequences (e.g., the viral origin of replication) and mediate strand-specific nicking to initiate replication.
As a recombinant protein, Rep78 has been extensively studied for its unique biological properties. Its ability to mediate site-specific integration into the human genome—particularly at chromosome 19q13.42 (AAVS1 locus)—has sparked interest in gene therapy applications. Researchers have explored Rep78 as a tool to enhance the safety of gene delivery systems by directing therapeutic transgenes to predefined genomic sites, minimizing random integration risks associated with insertional mutagenesis.
Beyond AAV biology, Rep78 has shown utility in modulating host-cell processes. It can inhibit proliferation of certain viruses (e.g., HPV, HIV) and oncoproteins, partly by interfering with host transcription factors. However, its cytotoxicity in mammalian cells and complex regulation pose challenges for therapeutic use. To address this, engineered Rep78 variants with reduced toxicity or enhanced specificity are under development.
Recombinant Rep78 is typically produced in *E. coli* or mammalian expression systems, purified via affinity chromatography, and validated for enzymatic activity. Its applications span gene editing, viral vector production, and studies on DNA repair mechanisms. Ongoing research aims to harness Rep78's unique functions to advance precision medicine and synthetic biology tools.
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