| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CELF1 |
| Uniprot No | Q92879 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-486aa |
| 氨基酸序列 | MNGTLDHPDQPDLDAIKMFVGQVPRTWSEKDLRELFEQYGAVYEINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNMKVLPGMHHPIQMKPADSEKNNAVEDRKLFIGMISKKCTENDIRVMFSSFGQIEECRILRGPDGLSRGCAFVTFTTRAMAQTAIKAMHQAQTMEGCSSPMVVKFADTQKDKEQKRMAQQLQQQMQQISAASVWGNLAGLNTLGPQYLALYLQLLQQTASSGNLNTLSSLHPMGGLNAMQLQNLAALAAAASAAQNTPSGTNALTTSSSPLSVLTSSGSSPSSSSSNSVNPIASLGALQTLAGATAGLNVGSLAGMAALNGGLGSSGLSNGTGSTMEALTQAYSGIQQYAAAALPTLYNQNLLTQQSIGAAGSQKEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMNGFQIGMKRLKVQLKRSKNDSKPY |
| 预测分子量 | 52kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CELF1重组蛋白的3篇代表性文献示例(基于研究领域常见方向，非真实文献)：
---
1. **文献名称**：*"Expression and purification of recombinant CELF1 protein for RNA-binding studies"*
**作者**：Smith A, et al.
**摘要**：本研究描述了一种在大肠杆菌中高效表达并纯化具有生物活性的重组CELF1蛋白的方法。通过亲和层析和离子交换层析获得高纯度蛋白，并利用凝胶迁移实验(EMSA)验证其与靶RNA的结合能力，为体外研究CELF1的RNA相互作用机制提供了工具。
---
2. **文献名称**：*"Structural analysis of CELF1 RRM domains reveals mechanisms of alternative splicing regulation"*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了重组CELF1蛋白的RNA识别基序(RRM)结构，揭示了其与特定RNA序列结合的分子机制。实验表明，CELF1的RRM2结构域在调控肌肉特异性基因选择性剪接中起关键作用，为强直性肌营养不良(DM1)的治疗提供了结构基础。
---
3. **文献名称**：*"Functional rescue of mis-splicing in myotonic dystrophy by recombinant CELF1 delivery"*
**作者**：Wang Y, et al.
**摘要**：研究利用重组CELF1蛋白在DM1细胞模型中纠正异常的RNA剪接。通过脂质体递送CELF1蛋白，恢复了胰岛素受体(IR)和肌肉氯离子通道(ClC-1)的正常剪接模式，证明补充功能性CELF1可能缓解DM1病理。
---
注：以上文献为示例性内容，实际文献需通过PubMed或Web of Science等平台检索关键词(如“CELF1 recombinant protein”)获取。
**Background of CELF1 Recombinant Protein**
CELF1 (CUGBP Elav-like family member 1), also known as CUG-BP1. is an RNA-binding protein belonging to the CELF family, which plays critical roles in post-transcriptional gene regulation. It is involved in RNA splicing, stability, translation, and decay by binding to specific sequences in target mRNAs. CELF1 is particularly notable for its role in developmental processes, muscle function, and cellular stress responses. Dysregulation of CELF1 has been linked to diseases such as myotonic dystrophy type 1 (DM1), where its aberrant activity contributes to mis-splicing of transcripts, and cancer, where it may influence tumor progression via mRNA metabolism.
Recombinant CELF1 protein is engineered for in vitro studies to dissect its molecular mechanisms. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the purified protein retains functional domains, including three RNA recognition motifs (RRMs) and a linker region critical for RNA binding and protein interactions. Researchers use recombinant CELF1 to analyze RNA-protein interactions, study splicing regulation in cell-free systems, or explore its phosphorylation-dependent activity. It also serves as a tool for screening potential therapeutics targeting CELF1-driven pathologies.
The availability of recombinant CELF1 has advanced understanding of its dual roles in normal physiology and disease, offering insights into RNA-centric regulatory networks and opportunities for translational research.
×
