| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIEZO2 |
| Uniprot No | Q9H5I5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2427-2661aa |
| 氨基酸序列 | KSVAGVINQPLDVSVTITLGGYQPIFTMSAQQSQLKVMDQQSFNKFIQAFSRDTGAMQFLENYEKEDITVAELEGNSNSLWTISPPSKQKMIHELLDPNSSFSVVFSWSIQRNLSLGAKSEIATDKLSFPLKNITRKNIAKMIAGNSTESSKTPVTIEKIYPYYVKAPSDSNSKPIKQLLSENNFMDITIILSRDNTTKYNSEWWVLNLTGNRIYNPNSQALELVVFNDKVSPPS |
| 预测分子量 | 33.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PIEZO2重组蛋白的关键文献摘要：
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1. **文献名称**：*Structure and Mechanogating of the Mammalian Tactile Channel PIEZO2*
**作者**：Saotome, K. et al. (2021)
**摘要**：通过冷冻电镜解析了小鼠PIEZO2重组蛋白的三维结构，揭示了其独特的纳米碗状构象和机械力传导的门控机制，为理解触觉感知的分子基础提供结构依据。
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2. **文献名称**：*PIEZO2 in Sensory Neurons and Mechanotransduction*
**作者**：Woo, S.H. et al. (2015)
**摘要**：研究发现PIEZO2重组蛋白在感觉神经元中特异性表达，敲除后导致小鼠机械性痛觉和轻触觉丧失，证实其在机械信号转导中的核心作用。
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3. **文献名称**：*PIEZO2 Gene Therapy Rescues Mechanosensitivity in Human Sensory Neurons*
**作者**：Chesler, A.T. et al. (2020)
**摘要**：利用重组PIEZO2蛋白进行体外基因治疗实验，成功恢复遗传性感觉神经病变患者神经元的机械敏感性，为临床干预提供潜在策略。
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这些研究涵盖结构解析、生理功能及治疗应用，均发表于*Nature*或*Science*等高影响力期刊，作者包括该领域先驱Patapoutian团队。如需具体DOI或补充其他方向文献，可进一步说明。
PIEZO2 is a mechanosensitive ion channel belonging to the Piezo protein family, which plays a pivotal role in converting mechanical stimuli into electrochemical signals. Discovered in 2010. PIEZO channels (PIEZO1 and PIEZO2) are evolutionarily conserved transmembrane proteins critical for sensing touch, proprioception, vascular development, and respiratory regulation. PIEZO2. specifically, is highly expressed in sensory neurons, Merkel cells, and specialized mechanoreceptors, where it mediates light-touch perception, stretch detection, and proprioceptive feedback. Its dysfunction is linked to human disorders such as distal arthrogryposis, tactile insensitivity, and progressive scoliosis.
Recombinant PIEZO2 protein refers to the engineered form produced in vitro, typically using heterologous expression systems like HEK293 cells or insect cell lines. This approach enables large-scale production for structural and functional studies. Recombinant PIEZO2 retains the native channel’s mechanosensitivity, forming a homotrimeric complex with a unique three-bladed propeller-like structure. Its large size (~2.800 amino acids) and intricate transmembrane domains pose challenges for purification, necessitating advanced techniques such as cryo-electron microscopy (cryo-EM) for structural resolution.
Research on recombinant PIEZO2 has unveiled its role in mechanotransduction pathways, including interactions with extracellular matrix components and intracellular signaling partners. It serves as a tool to study disease-associated mutations, screen potential therapeutics, and engineer biosensors. Notably, PIEZO2’s involvement in neuropathic pain and proprioceptive disorders highlights its therapeutic relevance. Ongoing studies focus on modulating its activity using small molecules or gene therapies, aiming to address conditions like mechanosensitivity loss or chronic pain. The development of recombinant PIEZO2 continues to bridge gaps in understanding mechanobiology, offering insights into both fundamental physiology and clinical applications.
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