| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | GRH; GNRH; HH12; LHRH; LNRH |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human GNRH1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CAST抗体的3-4篇虚构参考文献示例(基于学术研究常见方向，实际文献需通过数据库查询)：
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1. **文献名称**：*Calpastatin (CAST) Antibodies in Autoimmune Encephalitis: Diagnostic Utility and Pathogenic Implications*
**作者**：Smith J, et al.
**摘要**：本研究探讨了CAST抗体在自身免疫性脑炎患者中的检测价值，发现其与特定亚型的临床症状相关，提示其可能通过干扰神经元钙蛋白酶调控通路参与疾病发生。
2. **文献名称**：*Expression and Functional Analysis of CAST in Colorectal Cancer Metastasis*
**作者**：Wang L, et al.
**摘要**：通过免疫组化与体外实验，揭示了CAST在结直肠癌中的高表达与肿瘤侵袭性正相关，抑制CAST可降低钙蛋白酶活性并减缓癌细胞迁移。
3. **文献名称**：*Novel CAST Autoantibodies in Paraneoplastic Syndromes: A Multicenter Study*
**作者**：Garcia R, et al.
**摘要**：多中心研究鉴定了CAST抗体作为副肿瘤综合征的新型生物标志物，其与特定肿瘤类型(如小细胞肺癌)的关联为早期诊断提供依据。
4. **文献名称**：*Role of Calpastatin in Neurodegeneration: Insights from CAST Knockout Models*
**作者**：Kim S, et al.
**摘要**：利用CAST基因敲除小鼠模型，证明CAST缺失导致钙蛋白酶过度活化，加剧tau蛋白异常聚集，提示其在阿尔茨海默病中的潜在调控作用。
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**注**：以上内容为示例，实际文献需通过PubMed、Google Scholar等平台以“CAST antibody”“calpastatin autoantibody”等关键词检索。
**Background of CAST Antibodies**
CAST (Calpastatin) antibodies are essential tools in studying the regulation of calpain, a calcium-dependent protease involved in critical cellular processes like apoptosis, cell signaling, and cytoskeletal remodeling. Calpastatin, the endogenous inhibitor of calpain, binds to and blocks calpain activity, maintaining protease homeostasis. Structurally, calpastatin contains multiple inhibitory domains, allowing it to interact with various calpain isoforms.
CAST antibodies are widely used in research to detect calpastatin expression and distribution in tissues or cells via techniques such as Western blot, immunohistochemistry, and immunofluorescence. These antibodies help elucidate calpastatin's role in physiological and pathological contexts, including neurodegenerative diseases (e.g., Alzheimer’s), muscular dystrophy, and cardiovascular disorders, where calpain overactivation is implicated.
Available as monoclonal or polyclonal forms, CAST antibodies are typically raised in hosts like rabbits or mice, with specificity validated across species (human, mouse, rat). Their application extends to therapeutic research, as modulating calpain-calpastatin balance is a potential strategy for treating calpain-related pathologies. Understanding calpastatin's regulatory mechanisms through these antibodies provides insights into cellular stress responses and disease mechanisms.
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