| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRGM |
| Uniprot No | A1A4Y4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-181aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMKETLKIVSRTPVNITMAGDSGNGMSTFIS ALRNTGHEGKASPPTELVKATQRCASYFSSHFSNVVLWDLPGTGSATTTL ENYLMEMQFNRYDFIMVASAQFSMNHVMLAKTAEDMGKKFYIVWTKLDMD LSTGALPEVQLLQIRENVLENLQKERVCEY |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"IRGM regulates autophagy to mediate defense against tuberculosis"**
- **作者**: Kumar, D., et al.
- **摘要**: 研究IRGM重组蛋白通过调控自噬通路在宿主抗结核分枝杆菌感染中的作用，发现其通过促进自噬体成熟增强免疫清除能力。
2. **"Structural insights into the GTPase activity of IRGM and its interaction with autophagy machinery"**
- **作者**: Smith, J., et al.
- **摘要**: 解析IRGM重组蛋白的晶体结构，揭示其GTP酶活性对自噬相关蛋白(如LC3)的结合机制，阐明其在炎症调控中的分子基础。
3. **"IRGM-mediated autophagy promotes clearance of intracellular pathogens via inflammasome modulation"**
- **作者**: Singh, S.B., et al.
- **摘要**: 证明IRGM重组蛋白通过协调自噬与炎症小体活性，抑制沙门氏菌等胞内病原体的存活，并减轻过度炎症反应。
4. **"IRGM polymorphisms and recombinant protein therapy in inflammatory bowel disease"**
- **作者**: Lee, W., et al.
- **摘要**: 探讨IRGM基因多态性对克罗恩病易感性的影响，并评估重组IRGM蛋白在小鼠模型中缓解肠道炎症的潜在治疗价值。
(注：上述文献为示例，实际引用需根据具体研究检索验证。)
**Background of IRGM Recombinant Protein**
The Immunity-Related GTPase Family M (IRGM) is a member of the GTPase protein family, which plays a critical role in innate immunity, particularly in autophagy and host defense against intracellular pathogens. Initially identified for its homology to the murine Irgm proteins, human IRGM regulates autophagy by interacting with core machinery components like ULK1 and Beclin-1. facilitating the lysosomal degradation of invading microbes (e.g., *Mycobacterium tuberculosis*) and damaged organelles. Dysregulation of IRGM has been linked to autoimmune disorders (e.g., Crohn’s disease) and infectious diseases, highlighting its dual role in maintaining cellular homeostasis and immune responses.
IRGM recombinant protein is engineered through molecular cloning, typically expressed in bacterial or mammalian systems (e.g., *E. coli* or HEK293 cells) to ensure proper folding and post-translational modifications. Purification techniques, such as affinity chromatography, yield high-purity protein for functional studies. Researchers utilize IRGM recombinant proteins to dissect its molecular mechanisms, including GTPase activity, autophagy induction, and interactions with microbial or host proteins. These studies are pivotal for understanding disease pathogenesis and developing targeted therapies.
Recent advances also explore IRGM’s role in cancer and neurodegenerative diseases, broadening its therapeutic potential. Despite challenges in structural characterization due to its intrinsically disordered regions, IRGM remains a focal point in immunometabolism and infection biology research. Its recombinant form serves as a vital tool for drug screening and biomarker discovery, underscoring its translational relevance in precision medicine.
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