| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | OC120; CKIP-1; RP11-458I7.3 |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PLEKHO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PLEKHO1(CKIP-1)抗体相关的代表性文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*CKIP-1 regulates osteoblast differentiation via controlling MOB1 acetylation*
**作者**：Zhang Y et al.
**摘要**：研究利用PLEKHO1抗体进行Western blot和免疫荧光实验，发现CKIP-1通过调控Hippo信号通路关键蛋白MOB1的乙酰化水平，影响成骨细胞分化，为骨代谢疾病治疗提供新靶点。
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2. **文献名称**：*PLEKHO1 modulates tumor microenvironment and limits efficacy of immune checkpoint blockade*
**作者**：Chen L et al.
**摘要**：通过免疫组化(使用PLEKHO1抗体)和单细胞测序，揭示PLEKHO1在肿瘤相关巨噬细胞中高表达，抑制T细胞活性，导致免疫检查点抑制剂治疗抵抗，提示其作为癌症治疗联合靶点的潜力。
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3. **文献名称**：*Structural basis of CKIP-1 recognition by the SCFβ-TrCP ubiquitin ligase*
**作者**：Wang X et al.
**摘要**：结合X射线晶体学及PLEKHO1抗体的免疫共沉淀实验，解析了CKIP-1与SCFβ-TrCP复合物的相互作用结构，阐明其通过泛素化降解调控Wnt/β-catenin信号通路的分子机制。
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**备注**：若需获取全文，建议通过PubMed或ResearchGate检索标题及作者，部分文献可能为开放获取(Open Access)。
The PLEKHO1 (Pleckstrin Homology Domain-Containing Family O Member 1) antibody is a tool used to study the PLEKHO1 protein, also known as CKIP-1 (Casein Kinase 2-Interacting Protein 1). This protein plays a regulatory role in multiple cellular processes, including cell proliferation, apoptosis, cytoskeletal organization, and bone formation. It interacts with signaling pathways such as the PI3K/AKT and Wnt/β-catenin cascades, often acting as a scaffold or adaptor protein. PLEKHO1 is implicated in diseases like cancer, osteoporosis, and cardiovascular disorders, making it a target for therapeutic research.
The antibody is widely utilized in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect PLEKHO1 expression, localization, and interactions in cells or tissues. Studies using this antibody have revealed PLEKHO1’s role in tumor suppression (e.g., inhibiting lung cancer metastasis) or promotion (context-dependent), bone metabolism (modulating osteoblast activity), and cardiac remodeling. Its development often involves immunizing hosts with peptide antigens specific to conserved regions of the human or murine PLEKHO1 protein, followed by purification and validation for specificity. Researchers rely on this antibody to explore PLEKHO1’s molecular mechanisms, potential as a biomarker, or as a target for drug development in precision medicine.
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