| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITGB1 |
| Uniprot No | P05556 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-798aa |
| 氨基酸序列 | MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITGB1重组蛋白的3篇参考文献示例(内容基于虚构，仅供参考格式)：
1. **文献名称**：*Structural basis of integrin β1 activation for ligand binding*
**作者**：Lee, J. et al.
**摘要**：该研究通过X射线晶体学解析了ITGB1重组蛋白的构象变化，揭示了其与配体结合时的激活机制，为靶向整合素信号通路的药物设计提供结构基础。
2. **文献名称**：*ITGB1-mediated adhesion regulates cancer cell stemness and metastasis*
**作者**：Wei, Y. et al.
**摘要**：利用重组ITGB1蛋白进行功能实验，证明其通过激活FAK/Src信号通路增强肿瘤细胞的干性和转移能力，提示ITGB1作为癌症治疗的潜在靶点。
3. **文献名称**：*Recombinant ITGB1 protein modulates extracellular matrix remodeling in fibrosis*
**作者**：Smith, R. & García, A.
**摘要**：研究发现重组ITGB1蛋白通过结合纤连蛋白和胶原蛋白，调控成纤维细胞的ECM重塑过程，为纤维化疾病的干预策略提供新思路。
(注：以上文献为示例性内容，实际引用需查询真实数据库如PubMed或Web of Science。)
Integrin beta-1 (ITGB1), a key subunit of the integrin family, is a transmembrane cell adhesion receptor that mediates interactions between cells and the extracellular matrix (ECM). As part of heterodimeric complexes with various α subunits (e.g., α3. α4. α5), ITGB1 plays a critical role in regulating cell migration, proliferation, survival, and differentiation by facilitating bidirectional signaling across the plasma membrane. These processes are essential for embryogenesis, tissue repair, immune responses, and cancer progression. Dysregulation of ITGB1 is linked to pathological conditions, including fibrosis, metastatic cancers, and autoimmune disorders.
Recombinant ITGB1 protein is engineered using expression systems like mammalian cells (e.g., HEK293. CHO) to ensure proper post-translational modifications, such as glycosylation, which are vital for its structural and functional integrity. The purified protein typically retains functional domains, including the extracellular ligand-binding region, transmembrane segment, and cytoplasmic tail, enabling its use in studying integrin-ligand interactions (e.g., with fibronectin, laminin) or screening therapeutic agents targeting integrin-mediated pathways.
In research, recombinant ITGB1 serves as a tool to investigate cell-ECM adhesion mechanisms, mechanotransduction, and signaling cascades (e.g., FAK, MAPK pathways). It also aids in developing anti-cancer therapies or biomimetic scaffolds for regenerative medicine. Quality-controlled via SDS-PAGE, Western blot, and functional assays (e.g., cell adhesion inhibition), recombinant ITGB1 provides a reproducible platform for both basic and translational studies. Its applications extend to modeling diseases, drug discovery, and understanding microenvironmental cues influencing cellular behavior.
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