| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | JAM2 |
| Uniprot No | Q9BX67 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-241aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMVNLKSSNRTPVVQEFESVELSCIITD SQTSDPRIEWKKIQDEQTTYVFFDNKIQGDLAGRAEILGKTSLKIWNVTR RDSALYRCEVVARNDRKEIDEIVIELTVQVKPVTPVCRVPKAVPVGKMAT LHCQESEGHPRPHYSWYRNDVPLPTDSRANPRFRNSSFHLNSETGTLVFT AVHKDDSGQYYCIASNDAGSARCEEQEMEVYDLN |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于JAM2重组蛋白的3篇参考文献示例(注：文献为虚拟示例，仅供参考格式)：
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1. **标题**: *"Recombinant JAM2 Protein Modulates Endothelial Barrier Function in Inflammatory Responses"*
**作者**: Smith A, et al.
**摘要**: 研究通过大肠杆菌系统表达重组JAM2蛋白，发现其能够抑制促炎因子诱导的内皮细胞通透性增加，表明JAM2在维持血管屏障稳定性中起关键作用。
2. **标题**: *"Structural Characterization of JAM2 Extracellular Domain Using Recombinant Protein Expression"*
**作者**: Tanaka K, et al.
**摘要**: 利用昆虫细胞系统表达JAM2胞外域重组蛋白，通过X射线晶体学解析其三维结构，揭示了与配体结合的关键氨基酸位点，为靶向药物设计提供依据。
3. **标题**: *"JAM2 Recombinant Protein Attenuates Autoimmune Encephalomyelitis by Regulating T Cell Trafficking"*
**作者**: Müller R, et al.
**摘要**: 在小鼠模型中，注射重组JAM2蛋白显著减少中枢神经系统的T细胞浸润，缓解实验性自身免疫性脑脊髓炎症状，提示其作为多发性硬化症治疗的潜在策略。
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注：实际文献需通过PubMed、Web of Science等平台检索关键词(如"JAM2 recombinant"或"recombinant JAM2 function")获取。
**Background of JAM2 Recombinant Protein**
Junctional adhesion molecule 2 (JAM2), also known as JAM-B or VE-JAM, is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. It is characterized by two extracellular Ig-like domains (one V-type and one C2-type), a single transmembrane region, and a cytoplasmic tail involved in intracellular signaling. JAM2 plays a critical role in regulating cell-cell adhesion, barrier function, and leukocyte migration across endothelial or epithelial layers. It is expressed in vascular endothelial cells, lymphocytes, and certain epithelial tissues, where it mediates homophilic (JAM2-JAM2) or heterophilic (e.g., JAM2-JAM3) interactions.
The recombinant JAM2 protein is engineered using molecular cloning techniques, often expressed in mammalian systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. This purified protein retains the functional extracellular domains, enabling researchers to study JAM2's interactions with its ligands or receptors in vitro. For instance, JAM2 binds to integrins like α4β1 and αLβ2. modulating immune cell adhesion and trafficking. Its role in inflammatory responses, angiogenesis, and cancer metastasis has made it a focus in studies targeting autoimmune diseases, vascular disorders, and tumor biology.
Additionally, JAM2 recombinant proteins are utilized to develop therapeutic antibodies or inhibitors to block pathological interactions in conditions like multiple sclerosis or cancer. Research also explores its involvement in maintaining blood-brain barrier integrity and lymphatic system regulation. By providing a controlled and scalable source of functional JAM2. recombinant proteins facilitate mechanistic studies and drug discovery efforts aimed at modulating JAM2-mediated pathways.
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