| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KDSR |
| Uniprot No | Q06136 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-270aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMKPLALPGAHVVVTGGSSGIGKCIAIECYK QGAFITLVARNEDKLLQAKKEIEMHSINDKQVVLCISVDVSQDYNQVENV IKQAQEKLGPVDMLVNCAGMAVSGKFEDLEVSTFERLMSINYLGSVYPSR AVITTMKERRVGRIVFVSSQAGQLGLFGFTAYSASKFAIRGLAEALQMEV KPYNVYITVAYPPDTDTPGFAEENRTKPLETRLISETTSVCKPEQVAKQI VKDAIQGNFNSSLGSD |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KDSR重组蛋白的参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库验证)：
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1. **文献名称**: *Functional characterization of recombinant human KDSR: insights into sphingolipid metabolism*
**作者**: Smith J, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组人源KDSR蛋白，通过酶动力学分析证实其催化3-酮二氢鞘氨醇还原为二氢鞘氨醇的功能，并发现其活性依赖NADPH。研究为鞘脂代谢异常相关疾病的机制提供了分子基础。
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2. **文献名称**: *Crystal structure of KDSR reveals substrate binding and catalytic mechanism*
**作者**: Tanaka R, et al.
**摘要**: 通过X射线晶体学解析了重组KDSR蛋白的三维结构，揭示了其与底物和辅因子NADPH的结合模式，阐明了催化活性位点的关键氨基酸残基，为设计靶向KDSR的药物提供了结构依据。
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3. **文献名称**: *KDSR mutations disrupt epidermal ceramide synthesis: Rescue by recombinant protein delivery*
**作者**: Lee H, et al.
**摘要**: 研究报道了KDSR基因突变导致皮肤鱼鳞病的病例，通过体外重组KDSR蛋白递送至患者角质细胞，恢复了神经酰胺合成能力，证明了重组蛋白在治疗中的潜在应用价值。
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4. **文献名称**: *Heterologous expression of KDSR in yeast: A model for studying sphingolipid-related disorders*
**作者**: Garcia M, et al.
**摘要**: 在酵母系统中异源表达重组KDSR蛋白，发现其可互补酵母鞘脂代谢缺陷表型，建立了基于酵母的疾病模型，用于高通量筛选KDSR功能调控化合物。
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建议通过PubMed或Google Scholar以关键词“KDSR recombinant”“3-ketodihydrosphingosine reductase expression”检索最新文献。
**Background of KDSR Recombinant Protein**
KDSR (3-ketodihydrosphingosine reductase) is a key enzyme in the sphingolipid biosynthesis pathway, catalyzing the reduction of 3-ketodihydrosphingosine (KDS) to dihydrosphingosine (DHS). Sphingolipids are essential components of cell membranes and play critical roles in cellular signaling, apoptosis, and membrane integrity. Dysregulation of sphingolipid metabolism is linked to various diseases, including cancer, neurodegenerative disorders, and dermatological conditions.
The KDSR gene encodes a transmembrane protein localized to the endoplasmic reticulum. Mutations in KDSR are associated with congenital dermatological disorders, such as progressive symmetric erythrokeratoderma and severe skin fragility syndromes, highlighting its importance in epidermal homeostasis. Recombinant KDSR protein is produced using expression systems like *E. coli* or mammalian cells, enabling detailed study of its enzymatic activity, structure, and interactions.
Research applications of KDSR recombinant protein include elucidating mechanisms of sphingolipid metabolism, screening for inhibitors or activators to modulate pathway flux, and investigating disease-related mutations. Its role in generating bioactive sphingolipid intermediates, such as ceramides and sphingosine-1-phosphate, makes it a potential therapeutic target for conditions involving dysregulated lipid signaling.
Characterization of KDSR involves biochemical assays (e.g., NADPH-dependent reductase activity), structural analysis (X-ray crystallography or cryo-EM), and cell-based studies to explore its physiological and pathological relevance. Advances in recombinant protein technology continue to enhance understanding of KDSR's function, aiding drug discovery and therapeutic strategies targeting sphingolipid-related diseases.
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