| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PTAR3 |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RABGGTA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及RABGGTA抗体的参考文献及其摘要：
1. **"Rab geranylgeranyltransferase alpha subunit (RABGGTA) regulates vesicular transport in neuronal cells"**
- **作者**: Zhang Y, et al.
- **摘要**: 研究利用RABGGTA抗体验证其在神经元中的表达，发现RABGGTA缺失导致突触囊泡运输异常，提示其通过调控Rab蛋白异戊二烯化影响神经递质释放。
2. **"Characterization of RabGGTase subunits in cancer cell migration"**
- **作者**: Lee S, et al.
- **摘要**: 通过Western blot和免疫荧光(使用RABGGTA抗体)发现，RABGGTA在侵袭性癌细胞中高表达，敲低后抑制Rab蛋白膜定位，从而减少细胞迁移和转移。
3. **"Interaction between RABGGTA and VPS34 complex in autophagy regulation"**
- **作者**: Chen L, et al.
- **摘要**: 研究通过免疫共沉淀(RABGGTA抗体)证明RABGGTA与自噬相关蛋白VPS34存在相互作用，调控自噬小体形成，影响细胞应激反应。
4. **"Developmental role of RABGGTA in Drosophila melanogaster"**
- **作者**: Gupta A, et al.
- **摘要**: 在果蝇模型中利用RABGGTA抗体检测其时空表达，发现其缺失导致胚胎发育缺陷，强调其对Rab依赖性膜运输的保守调控作用。
(注：以上文献为虚拟示例，实际引用需查询真实数据库如PubMed。)
The RABGGTA antibody is designed to detect the alpha subunit of Rab geranylgeranyltransferase (Rab GGTase), a critical enzyme complex involved in post-translational lipid modification of Rab GTPases. Rab proteins, key regulators of intracellular vesicle trafficking, require geranylgeranylation for membrane association and function. The Rab GGTase complex, composed of RABGGTA (catalytic subunit) and RABGGTB (substrate-binding subunit), facilitates the attachment of geranylgeranyl groups to Rab proteins. This modification is essential for Rab-mediated processes like vesicle formation, transport, and fusion, which underpin cellular secretion, autophagy, and receptor recycling.
RABGGTA antibodies are widely used in research to study Rab protein prenylation, enzyme complex assembly, and their roles in cellular trafficking pathways. They enable detection of RABGGTA expression via techniques such as Western blotting, immunofluorescence, and immunoprecipitation. Dysregulation of Rab GGTase activity has been linked to diseases, including cancer and neurodegenerative disorders, making these antibodies valuable tools for investigating pathological mechanisms or therapeutic targets.
Developed in hosts like rabbits or mice, RABGGTA antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Their applications span basic cell biology, cancer research, and studies of membrane dynamics, highlighting their importance in understanding Rab-mediated regulatory networks.
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