Rabbit Polyclonal VARS1 Antibody

中文名： VARS1抗体
别名： G7A; VARS; VARS2; NDMSCA

货号: IPDX03601

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/50-1/100	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	1/5000-1/10000	Human,Mouse,Rat

产品详情

Aliases	G7A; VARS; VARS2; NDMSCA
WB Predicted band size	140 kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Fusion protein of human VARS1
Formulation	Purified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Ramos cell lysate, Primary antibody: P03601(VARS1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 5 seconds
The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P03601(VARS1 Antibody) at dilution 1/50. (Original magnification: ×200)

参考文献

以下是关于VARS1抗体的模拟参考文献示例(实际文献可能需要通过学术数据库查询确认)：

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1. **标题**：*VARS1 mutations and antibody validation in human neurological disorders*

**作者**：Sistermans EA, et al.

**摘要**：本研究利用VARS1特异性抗体，通过免疫印迹和免疫组化技术，揭示了VARS1基因突变患者脑组织中缬氨酰-tRNA合成酶表达显著降低，证实其与婴儿期起病的脑病相关。

2. **标题**：*Mitochondrial localization of VARS1 and its functional implications*

**作者**：Nafisinia M, et al.

**摘要**：通过亚细胞分离联合VARS1抗体检测，发现VARS1蛋白在线粒体中富集，提示其在线粒体tRNA缬氨酸加载中的关键作用，突变可能导致能量代谢异常。

3. **标题**：*Antibody-based detection of VARS1 variants in diagnostic workflows*

**作者**：Ramos-Quiroga C, et al.

**摘要**：比较了多种商业VARS1抗体的特异性，筛选出高灵敏度的抗体用于ELISA检测，为携带VARS1致病性变异的患者提供了快速诊断工具。

4. **标题**：*Therapeutic rescue of VARS1 dysfunction in a zebrafish model*

**作者**：Mendes MI, et al.

**摘要**：在斑马鱼模型中，使用反义寡核苷酸治疗后，通过VARS1抗体检测到蛋白表达恢复，为靶向治疗提供了实验依据。

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**注意**：以上内容为模拟示例，实际文献需查阅PubMed、Google Scholar等平台。建议结合关键词“VARS1 antibody”、“VARS1 encephalopathy”进一步检索。

背景信息

VARS1 antibodies target valyl-tRNA synthetase (VARS1), an enzyme critical for protein synthesis. As a member of the aminoacyl-tRNA synthetase (ARS) family, VARS1 catalyzes the attachment of valine to its cognate tRNA, ensuring accurate translation of genetic codes into proteins. Unlike some cytosolic ARS enzymes, VARS1 localizes to mitochondria, reflecting its specialized role in mitochondrial translation and energy production.

Mutations in the *VARS1* gene are linked to mitochondrial disorders, including neurodevelopmental impairments, encephalopathy, and multisystemic dysfunction. VARS1 antibodies are essential tools in research to study these pathologies, enabling detection of protein expression, subcellular localization, and interactions. They are used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to explore VARS1’s role in mitochondrial health and disease mechanisms.

Clinically, VARS1 antibodies may aid in diagnosing genetic disorders by identifying aberrant protein levels or mutations. Additionally, autoantibodies against VARS1 (part of anti-synthetase syndrome) are associated with autoimmune conditions like dermatomyositis, though this differs from research-grade antibodies targeting the enzyme itself. Understanding VARS1’s structure-function relationships and dysregulation continues to inform therapeutic strategies for mitochondrial and autoimmune diseases.