| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | DI1, DIR, NDI, V2R, ADHR, DIR3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human AVPR2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于AVPR2抗体的3篇参考文献示例(内容为模拟概括，非真实文献)：
---
1. **文献名称**: "Characterization of AVPR2 mutations in congenital nephrogenic diabetes insipidus using a novel antibody-based assay"
**作者**: Oueslati M, et al.
**摘要**: 本研究开发了一种特异性抗AVPR2抗体，用于检测X连锁遗传性肾源性尿崩症(NDI)患者的受体突变。通过免疫荧光和Western blot分析，发现部分突变导致AVPR2蛋白错误折叠并滞留于内质网，揭示了其致病机制。
2. **文献名称**: "Development of a sensitive ELISA for detecting AVPR2 autoantibodies in autoimmune-related diabetes insipidus"
**作者**: Saito T, et al.
**摘要**: 研究建立了一种基于AVPR2抗体的ELISA检测方法，用于识别自身免疫性疾病患者中的抗AVPR2自身抗体。临床样本分析表明，部分特发性尿崩症患者存在针对AVPR2的自身免疫反应，为诊断提供了新工具。
3. **文献名称**: "Structural insights into AVPR2 signaling through antibody-mediated crystallization"
**作者**: Thibonnier M, et al.
**摘要**: 利用单克隆抗体稳定AVPR2的构象，首次解析了该受体与抗利尿激素结合的晶体结构。研究揭示了受体激活的关键表位，为靶向AVPR2的药物设计提供了结构基础。
---
*注：以上文献为示例，实际引用需检索PubMed/Google Scholar等数据库获取真实信息。*
The arginine vasopressin receptor type 2 (AVPR2) is a G protein-coupled receptor (GPCR) primarily expressed in the renal collecting duct cells. It plays a critical role in regulating water homeostasis by mediating the antidiuretic effects of arginine vasopressin (AVP). Upon AVP binding, AVPR2 activates the Gs-adenylyl cyclase-cAMP pathway, triggering the insertion of aquaporin-2 (AQP2) water channels into the apical membrane, enabling water reabsorption. Dysregulation of AVPR2 is linked to X-linked nephrogenic diabetes insipidus (NDI), a condition characterized by impaired urinary concentrating ability due to mutations in the AVPR2 gene.
AVPR2 antibodies are essential tools for studying receptor expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry to detect AVPR2 in tissues or cultured cells. Researchers employ these antibodies to investigate AVPR2-related pathologies, including NDI, or to explore its role in disorders involving fluid imbalance, such as heart failure and hypertension. Additionally, AVPR2 antibodies aid in drug development targeting water retention disorders or AVP signaling pathways. Specificity and validation (e.g., knockout controls) are crucial for ensuring reliable results, as cross-reactivity with other GPCRs may occur. Commercial AVPR2 antibodies are typically raised against epitopes in extracellular or intracellular domains, with applications spanning basic research and clinical diagnostics.
×
