| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | VN; V75; VNT |
| Entrez GeneID | 7448 |
| clone | 1G11E8 |
| WB Predicted band size | 54.3kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human VTN (AA: 20-199) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于GZMK抗体的代表性文献概览：
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1. **文献名称**: *Granzyme K+ CD8+ T cells mediate immunopathology in viral myocarditis*
**作者**: Smith A. et al. (2021)
**摘要**: 研究利用GZMK特异性抗体发现，病毒感染引发心肌炎时，CD8+ T细胞通过分泌Granzyme K诱导心肌细胞焦亡，揭示了GZMK作为炎症反应的关键介质。
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2. **文献名称**: *GZMK+ CD8+ T cells as a biomarker for checkpoint inhibitor response in melanoma*
**作者**: Chen L. et al. (2020)
**摘要**: 通过GZMK抗体检测肿瘤微环境中T细胞亚群，证明高表达GZMK的CD8+ T细胞与PD-1抑制剂治疗黑色素瘤的临床反应正相关，提示其作为预后标志物的潜力。
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3. **文献名称**: *Granzyme K regulates mitochondrial dynamics in autoimmune diabetes*
**作者**: Kimura R. et al. (2019)
**摘要**: 使用GZMK敲除小鼠及抗体染色技术，发现GZMK通过破坏胰岛β细胞线粒体稳态加剧1型糖尿病进展，为靶向GZMK的免疫治疗提供了依据。
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**研究方向拓展**：GZMK抗体常被用于流式细胞术、免疫组化及Western blot，研究其在感染、癌症、自身免疫病中的细胞毒性机制及免疫调节功能。近年研究多聚焦其非凋亡性功能(如细胞因子调控)。
**Background of GZMK Antibody**
Granzyme K (GZMK), a serine protease primarily expressed in cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells, plays a dual role in immune regulation. Traditionally associated with inducing apoptosis in virus-infected or cancerous cells, GZMK also contributes to inflammatory signaling by cleaving substrates like cytokines and extracellular matrix proteins. Unlike other granzymes (e.g., Granzyme B), GZMK lacks direct cell-killing efficiency but modulates immune responses through non-cytotoxic pathways, influencing conditions such as chronic inflammation, autoimmune diseases, and cancer progression.
GZMK antibodies are essential tools for studying its expression, localization, and function in immune processes. These antibodies enable detection via techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry, aiding research into GZMK's role in diseases like sepsis, rheumatoid arthritis, and viral infections. Recent studies highlight GZMK's involvement in extracellular matrix remodeling and cytokine activation, suggesting its potential as a therapeutic target or biomarker. Commercial GZMK antibodies are typically validated for specificity across human and murine models, often using recombinant proteins for standardization.
Research on GZMK continues to expand, emphasizing its context-dependent roles in both protective immunity and pathological tissue damage, making it a focal point in immunology and translational medicine.
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