| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | HMG-R, HMGIY, HMGA1A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human HMGA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HMGA1抗体的3篇参考文献及其摘要概括：
1. **"HMGA1 is a novel biomarker of mesenchymal cancer stem cells in glioblastoma"**
- **作者**: Malek A, et al.
- **摘要**: 该研究通过免疫组织化学(使用HMGA1抗体)证明，HMGA1在胶质母细胞瘤的间充质型肿瘤干细胞中高表达，与肿瘤侵袭性和预后不良相关，提示其作为治疗靶点的潜力。
2. **"HMGA1 overexpression in colorectal carcinoma: A novel biomarker for tumor progression"**
- **作者**: Belton A, et al.
- **摘要**: 利用HMGA1特异性抗体进行Western blot和免疫组化分析，发现结直肠癌中HMGA1蛋白水平显著升高，且与转移和化疗耐药性相关，表明其可作为预后标志物。
3. **"HMGA1 regulates EMT and stemness in pancreatic cancer through Wnt/β-catenin pathway"**
- **作者**: Li Y, et al.
- **摘要**: 通过HMGA1抗体介导的染色质免疫沉淀(ChIP)和功能实验，揭示HMGA1通过激活Wnt通路促进胰腺癌细胞上皮间质转化(EMT)和干细胞特性，驱动肿瘤进展。
4. **"Antibody-based detection of HMGA1 in breast cancer reveals association with triple-negative subtype"**
- **作者**: Fedele M, et al.
- **摘要**: 研究采用HMGA1抗体对乳腺癌组织进行检测，发现HMGA1在三阴性乳腺癌中高表达，并与患者生存率降低相关，提示其在该亚型中的潜在诊断价值。
以上文献均涉及HMGA1抗体在肿瘤研究中的具体应用，涵盖表达分析、机制探索及临床关联性。
**Background of HMGA1 Antibody**
HMGA1 (High Mobility Group A1) is a non-histone chromatin-associated protein involved in regulating gene expression by modulating DNA structure and facilitating transcriptional complexes. It plays a critical role in cellular processes like proliferation, differentiation, and apoptosis. Dysregulation of HMGA1 is linked to various cancers, where its overexpression correlates with tumor progression, metastasis, and poor prognosis. It also associates with metabolic disorders, such as diabetes, and developmental abnormalities.
HMGA1 antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP) to detect HMGA1 in tissue samples or cell lines. Researchers employ them to explore HMGA1's role in oncogenesis, epithelial-mesenchymal transition (EMT), and therapeutic resistance. Validating antibody specificity is crucial due to HMGA1's structural similarities with HMGA2 and potential post-translational modifications.
Understanding HMGA1 dynamics through these antibodies aids in identifying biomarkers for cancer diagnosis and assessing its potential as a therapeutic target. Ongoing research focuses on clarifying HMGA1's mechanisms in disease and leveraging antibodies for diagnostic or functional studies.
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