| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/15-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5 |
| WB Predicted band size | 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PRMT5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRMT5抗体的3篇代表性文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*PRMT5-mediated methylation of histone H4R3 recruits DNMT3A, coupling histone and DNA methylation in gene silencing*
**作者**：Zhao Q. et al.
**摘要**：该研究利用PRMT5特异性抗体，通过染色质免疫沉淀(ChIP)和质谱分析，揭示了PRMT5催化组蛋白H4R3对称二甲基化，并招募DNA甲基转移酶DNMT3A，从而协同调控基因沉默的表观遗传机制。
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2. **文献名称**：*Therapeutic targeting of PRMT5-mediated SREBP1 methylation promotes c-Myc degradation in B-cell lymphoma*
**作者**：Huang T. et al.
**摘要**：研究通过Western blot和免疫组化(IHC)使用PRMT5抗体，证明PRMT5通过甲基化SREBP1调控c-Myc稳定性，靶向抑制PRMT5可诱导B细胞淋巴瘤凋亡，为治疗提供新策略。
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3. **文献名称**：*Development and validation of a PRMT5-specific monoclonal antibody for cancer biomarker analysis*
**作者**：Smith J. et al.
**摘要**：该文献报道了一种高特异性PRMT5单克隆抗体的开发与验证，通过ELISA、免疫荧光和流式细胞术证实其在多种癌症组织中的灵敏度和特异性，适用于临床样本的PRMT5表达定量检测。
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如需更详细文献信息(如期刊名称、年份等)，可进一步补充关键词或研究背景。
PRMT5 (Protein Arginine Methyltransferase 5) is a key enzyme responsible for symmetric dimethylation of arginine residues (SDMA) on histones and non-histone proteins, playing a critical role in epigenetic regulation, RNA splicing, DNA repair, and cellular signaling. As a member of the PRMT family, it catalyzes the transfer of methyl groups from S-adenosylmethionine (SAM) to specific arginine residues, primarily targeting substrates like histone H4R3. H3R8. and non-histone proteins (e.g., spliceosome components). Dysregulation of PRMT5 is linked to cancer, cardiovascular diseases, and neurological disorders, with overexpression observed in multiple malignancies, making it a therapeutic target.
PRMT5 antibodies are essential tools for studying its expression, localization, and functional roles. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC) to detect PRMT5 protein levels or assess methylation status of its substrates. Specificity varies depending on the epitope; some antibodies recognize total PRMT5. while others distinguish between methylated forms (e.g., anti-SDMA antibodies). Validated PRMT5 antibodies help elucidate its interaction networks, downstream pathways, and responses to inhibitors in preclinical models. Recent research focuses on developing selective PRMT5 inhibitors, and corresponding antibodies are critical for pharmacodynamic biomarker analysis in drug development. Challenges include cross-reactivity with other PRMTs, emphasizing the need for rigorous validation using knockout controls or peptide competition assays.
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