| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | CD90 |
| Entrez GeneID | 7070 |
| clone | 7E1B11 |
| WB Predicted band size | 18kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Purified recombinant fragment of human THY1 (AA: 17-132) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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1. **文献名称**: "MAP3K4调控胚胎发育中的细胞迁移机制研究"
**作者**: Smith J, et al.
**摘要**: 本研究通过Western blot和免疫荧光技术，利用MAP3K4抗体证实该激酶在小鼠胚胎神经管闭合阶段调控细胞迁移，揭示其磷酸化状态对下游信号通路的影响。
2. **文献名称**: "MAP3K4在卵巢癌中的表达与化疗耐药性关联分析"
**作者**: Zhang L, et al.
**摘要**: 采用免疫组化及免疫沉淀技术，通过MAP3K4抗体检测发现，卵巢癌组织中MAP3K4高表达与铂类化疗耐药性显著相关，提示其作为潜在治疗靶点。
3. **文献名称**: "MAP3K4-CBP复合物在炎症反应中的表观遗传调控作用"
**作者**: Brown K, et al.
**摘要**: 研究通过ChIP-seq和Co-IP实验结合MAP3K4抗体，揭示MAP3K4与组蛋白乙酰转移酶CBP形成复合物，调控巨噬细胞中促炎因子基因的染色质重塑。
4. **文献名称**: "CRISPR筛选发现MAP3K4缺失导致乳腺癌细胞代谢重编程"
**作者**: Wang Y, et al.
**摘要**: 利用MAP3K4抗体进行蛋白质印迹验证，发现敲低MAP3K4后乳腺癌细胞糖酵解通路异常激活，表明其在维持代谢稳态中的关键作用。
The MAP3K4 (Mitogen-Activated Protein Kinase Kinase Kinase 4), also known as MEKK4. is a serine/threonine kinase that plays a critical role in cellular stress response, proliferation, differentiation, and apoptosis. It is part of the MAPK signaling cascade, where it activates downstream kinases like MAP2K4/7. subsequently regulating JNK and p38 pathways. MAP3K4 is involved in diverse physiological processes, including embryonic development, cell migration, and inflammatory responses. Dysregulation of MAP3K4 has been linked to cancers, neurodegenerative disorders, and cardiovascular diseases, highlighting its importance in both normal and pathological states.
Antibodies targeting MAP3K4 are essential tools for studying its expression, localization, and function. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to detect MAP3K4 protein levels in tissues or cell lines. Researchers rely on specificity validation (e.g., knockout controls) to ensure accurate detection, as MAP3K4 shares structural similarities with other kinases. Commercial MAP3K4 antibodies are available in various forms, including monoclonal (raised in mice or rabbits) and polyclonal versions, each optimized for specific applications.
Understanding MAP3K4's role through antibody-based research provides insights into its dual regulatory mechanisms in signaling pathways and its potential as a therapeutic target. Such studies contribute to unraveling disease mechanisms and developing targeted therapies for conditions associated with MAP3K4 dysfunction.
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